Teton™ CytoProfiling

Single cell and spatial co-detection of RNA, protein, and cell morphology at subcellular resolution with no library prep and next-day results

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Cellular decoding unlocked

Every layer of biology – from cell morphology to RNA and protein expression – reveals critical insights into cell function. Teton CytoProfiling enables precise single cell spatial imaging of RNA, proteins, phospho-proteins, and cell morphology across hundreds of thousands to millions of cells with less than 1 hour of sample preparation and next-day results. From performing drug screens to resolving cell lineage trajectories, Teton unlocks single cell spatial multiomic analysis at scale.

Rich morphology and multiomic readouts

Morphology
RNA detection
Protein detection
DISS

Morphology

  • Visualize nucleus, cell membrane, actin, endoplasmic reticulum, Golgi apparatus, and mitochondria
  • Automated, onboard detection by ABC sequencing
  • Multiplex feature detection with reversible cell labels
  • Sequential RNA and protein imaging with low background
  • Real-time segmentation and morphology feature extraction with onboard primary analysis

RNA detection

  • Profile 350 transcripts with subcellular resolution
  • Less than 1 hour off-instrument workflow with automated onboard probe hybridization, amplification, and detection
  • Next-day run times with ABC sequencing of transcript-specific barcodes
  • Enhanced sensitivity and dynamic range with detection of 1000s transcripts/cell
  • Flexible panel options with pre-designed or custom configurations*

*Coming in 2025

Protein detection

  • Map 50 cell surface, intracellular, and phospho proteins at subcellular spatial resolution
  • Co-detect protein and RNA in same sample with optimized chemistries
  • Automated onboard antibody binding, amplification, and detection by ABC sequencing
  • Choose from pre-designed panels or custom options* with no conjugation or barcoding required

Direct In Sample Sequencing (DISS)

  • Unlock targeted and untargeted RNA sequencing at subcellular spatial resolution
  • Up to 100 cycles of sequencing with low phasing or pre-phasing errors
  • High on-target coverage for targeted sequencing applications
  • Enables unbiased 3’ sequencing, in situ CRISPR guide sequencing, expressed mutation detection, and more!

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How does it work?

Cell Preparation

Sample Preparation (<1 hour)

Sequencing + Primary Analysis (1 day)

Data Visualization

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See how Teton Chemistry Enables Multiomic Detection

Designed for your science

Sample types and segmentation models

Teton CytoProfiling is compatible with diverse sample types from adherent cells to cell suspension to primary cells or cell lines. With advanced segmentation models, you can get high-quality data for cell surface and intracellular markers in cell types ranging from immune to neural cell lines.

Cell Suspensions
Cell Suspensions
Adherent Cells
Adherent Cells

Pre-designed panels

Teton panels are available as curated, supported panels containing biologically relevant targets for a specific research area. Each panel is designed for the co-detection of cell typing markers, signaling molecules, and important drivers of cell function in normal and disease states.

MAPK Cell Cycle
MAPK Cell Cycle

MAP Kinase Signaling
Cell cycle regulation
Transcription
DNA replication

View target list →

MAPK Apoptosis
MAPK Apoptosis

MAP Kinase Signaling
Pro- and anti-apoptosis
Transcription
Stress response

View target list →

Immuno Oncology
Immuno Oncology

Immunophenotyping
Immune Response
Checkpoint Inhibitors
Hematology Oncology research

View target list →

Neuroscience
Neuroscience

Neurodifferentiation
Neurotransmission & Synaptic function
Neurodegeneration
Neuroinflammation

View target list →

Optimization reagents

Cell culture optimization: Test your cell culture conditions before committing to a full AVITI24 run. Simply plate cells, perform cell paint, assemble the flow cell, and visualize on either an AVITI24 or a microscope.

Antibody screening kit: Screen antibodies for use in a custom antibody panel. Couple your primary antibody of interest to a Teton detection probe, apply to cells, assemble the flow cell and read-out on your AVITI24—in less than 7 hours, you can determine antibody performance in a Teton assay.

Flexible protein customization

With Teton, we provide flexible paths to protein customization:

Custom add-on panels: Enables users to profile up to 88 unique primary antibodies coupled with our Teton detection probes. A simple workflow requires no chemical cross-linking and reduces time to generate a fully custom panel from months to hours.

Teton Focus Panels: Pre-designed sets of 24 target probes across immunology, oncology, neuroscience, and cell cycle research areas. Users can mix and match up to 3 focus panels per run or combine 2 focus panels with up to 24 custom antibodies.

View target list →

Ordering Information

Teton Panel Kits & Reagents
Teton Cartridge & Reagent Kit860-00039
Teton Human Neuro Panel Kit830-00038
Teton Human Immuno Panel Kit830-00039
Teton Human MAPK & Cell Cycle Panel Kit830-00040
Teton Human MAPK & Apoptosis Panel Kit830-00041
Protein Add-Ons
Teton Custom Add-On Protein Panel Assembly Kit860-00036
Teton Focus Protein Panel, Set 1, Cytokine Signaling830-00046
Teton Focus Protein Panel, Set 2, T Cell Activation830-00047
Teton Focus Protein Panel, Set 3, Innate Immunity830-00048
Teton Focus Protein Panel, Set 1, Cell Metabolism830-00049
Teton Focus Protein Panel, Set 2, Cell Stress & Apoptosis830-00050
Teton Focus Protein Panel, Set 3, Gene Regulation830-00051
Teton Focus Protein Panel, Set 1, Neurodegeneration830-00052
Teton Focus Protein Panel, Set 2, Neurodevelopment830-00053
Teton Focus Protein Panel, Set 3, Neuroinflammation830-00054
Teton Diversity Spike-In830-00043
Teton Custom Add-On Protein Buffer830-00044
Optimization and Screening Kits
Teton Cell Paint Probe Kit830-00045
Teton Onboard Cell Paint Imaging Kit860-00047
Teton Custom Antibody Screening Kit860-00035
Teton Slide Kits
Teton Slide Kit, PLL – 48 Well (2-pack)860-00041
Teton Slide Kit, Uncoated – 48 Well (2-pack)860-00042
Teton Slide Kit, PLL – 12 Well (2-pack)860-00031
Teton Slide Kit, Uncoated – 12 Well (2-pack)860-00032
Teton Slide Kit, PLL – 1 Well (2-pack)860-00029
Teton Slide Kit, Uncoated – 1 Well (2-pack)860-00030
Teton Accessories
Teton Flow Cell Assembly Tool Set860-00033
Teton Slide Kit Tool810-00021
Teton Slide Kit Tray (2-Pack)860-00044
Teton Flow Cell Assembly Kits860-00033
Teton, Flow Cell Assembly Kit, 1-Well or 48 Well (2-pack)860-00027
Teton, Flow Cell Assembly Kit, 12-Well (2-pack)860-00028

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