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  2. CytoProfiling

Teton™ CytoProfiling

Single cell and spatial co-detection of RNA, protein, and cell morphology at subcellular resolution with no library prep and next-day results

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Cellular decoding unlocked

Every layer of biology – from cell morphology to RNA and protein expression – reveals critical insights into cell function. Teton CytoProfiling enables precise single cell spatial imaging of RNA, proteins, phospho-proteins, and cell morphology across hundreds of thousands to millions of cells with less than 1 hour of sample preparation and next-day results. From performing drug screens to resolving cell lineage trajectories, Teton unlocks single cell spatial multiomic analysis at scale.

Rich morphology and multiomic readouts

Morphology
RNA detection
Protein detection
DiSS

Morphology

  • Visualize nucleus, cell membrane, actin, endoplasmic reticulum, Golgi apparatus, and mitochondria
  • Automated, onboard detection by ABC sequencing
  • Multiplex feature detection with reversible cell labels
  • Sequential RNA and protein imaging with low background
  • Real-time segmentation and morphology feature extraction with onboard primary analysis

RNA detection

  • Profile 350 transcripts with subcellular resolution
  • Less than 1 hour off-instrument workflow with automated onboard probe hybridization, amplification, and detection
  • Next-day run times with ABC sequencing of transcript-specific barcodes
  • Enhanced sensitivity and dynamic range with detection of 1000s transcripts/cell
  • Flexible panel options with pre-designed or custom configurations*

*Coming in 2025

Protein detection

  • Map 50 cell surface, intracellular, and phospho proteins at subcellular spatial resolution
  • Co-detect protein and RNA in same sample with optimized chemistries
  • Automated onboard antibody binding, amplification, and detection by ABC sequencing
  • Choose from pre-designed panels or custom options* with no conjugation or barcoding required

Direct in sample sequencing (DiSS)

  • Unlock targeted and untargeted RNA sequencing at subcellular spatial resolution
  • Up to 100 cycles of sequencing with low phasing or pre-phasing errors
  • High on-target coverage for targeted sequencing applications
  • Enables unbiased 3’ sequencing, in situ CRISPR guide sequencing, expressed mutation detection, and more!

*Coming in 2025

How does it work?

Cell Preparation

Sample Preparation (<1 hour)

Sequencing + Primary Analysis (1 day)

Data Visualization

Cytoprofiling teton Detection 2024 12 04 214709 oovv

See how Teton Chemistry Enables Multiomic Detection

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Designed for your science

Teton panels are available as curated, supported panels containing biologically relevant targets for a specific research area. Each panel is designed for the co-detection of cell typing markers, signaling molecules, and important drivers of cell function in normal and disease states.

Sample Compatibility

  • Immortalized cell lines
  • Primary cell lines
  • Co-cultures
  • Cell suspensions

Available pre-designed panels

MAPK panels 
Comprehensive critical signaling cascades involved in cell cycle regulation, transcription, DNA replication, stress response, and apoptosis.

Immuno-oncology panel
Co-detect immune cell markers, cytokines, signaling molecules, and biologically relevant targets involved in inflammation, innate and adaptive immunity, antiviral defense, and immune checkpoints. 

View list of targets > 

Neuroscience Panel
Markers to study neurodegenerative disorders, like Alzheimer’s and Parkinson’s disease, psychiatric disorders, and neurological cancers. 

View list of targets > 

Custom panels
Add customized protein panels tailored to your specific experimental needs with the Teton Custom Add-On Protein Panel Assembly Kit. In this workflow, user-supplied primary antibodies are coupled to Teton detection probes and added to any fixed panel at run time, reducing the time to generate a fully custom panel from months to hours.

Ordering Information

Teton Panel & Reagents
Teton Cartridge & Reagent Kit- 1 well860-00037
Teton Cartridge & Reagent Kit- 12 well860-00038
Teton Panel Kits
Teton Human Neuro Panel Kit830-00038
Teton Human Immuno Panel Kit830-00039
Teton Human MAPK & Cell Cycle Panel Kit830-00040
Teton Human MAPK & Apoptosis Panel Kit830-00041
Custom Protein Add-On
Teton Custom Add-On Protein Panel Assembly Kit860-00036
Teton Diversity Spike-In830-00043
Teton Optimization Kit
Teton Optimization Kit860-00022
Teton Slide Kits
Teton Slide Kit, PLL – 12 Well (2-pack)860-00031
Teton Slide Kit, Uncoated – 12 Well (2-pack)860-00032
Teton Slide Kit, PLL – 1 Well (2-pack)860-00029
Teton Slide Kit, Uncoated – 1 Well (2-pack)860-00030
Teton Flow Cell Assembly Tool Set
Teton Flow Cell Assembly Tool Set860-00033
Teton Flow Cell Assembly Kits
Teton, Flow Cell Assembly Kit, 1-Well (2-pack)860-00027
Teton, Flow Cell Assembly Kit, 12-Well (2-pack)860-00028

Resources

Download Brochure
Read Blog Post
Download Spec Sheet

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