Turn every perturbation into a multidimensional map

See more from every CRISPR screen: morphology, protein localization, and transcriptomics, all from the same cell.

CRISPR Screening with Element Biosciences

Link genetic perturbations to deep biological insight

CRISPR screening systematically perturbs genes to uncover how they shape cellular function. By linking genetic changes to phenotype, researchers can identify targets, define mechanisms, and reveal drivers of disease.

	Target discovery Uncover genes driving disease-relevant phenotypes at scale.		Target validation Confirm target function across molecular and cellular context.
	Mechanism of action Define how genetic perturbations reshape cellular pathways and function.		Genotype-to-phenotype linkage Map effects of variants with molecular and cellular readouts.
	Cell state and fate mapping Discover genetic regulators of differentiation and lineage commitment.		Resistance mapping Identify vulnerabilities that prevent or overcome resistance.

See the power of multimodal pooled screens with Cytos

	GUIDE DETECTION Confidently assign perturbations into single cells Direct In Sample Sequencing resolves 10s–10,000s of guide RNAs directly in cells. Simply design a single primer pair for your guide library, add it to your Cytos cartridge, and let onboard base calling and cell assignment streamline analysis.
	MORPHOLOGY Link genetic perturbations to mophological phenotypes Profile up to 24 subcellular structures with off-the-shelf and custom protein panels. Quantify morphology to reveal how perturbations reshape cell phenotype, from cytoskeletal reorganization to Golgi fragmentation.
	TRANSCRIPTOME Reveal transcriptome-wide effects of every perturbation Direct In Sample Sequencing unlocks 3’ transcriptome profiling to capture unbiased gene expression and reveal how each perturbation reshapes pathways, cell states, and broader transcriptional programs.
	PROTEIN Visualize protein localization changes Rich, multiplexed immunofluorescence-based protein read-outs reveals how genetic perturbations impact protein abundance, localization, and activation.

From cells to answers


Introduce CRISPR library Guide library delivery, selection, & treatments	Culture and fix cells Culture cells directly on AVITI24 slides & perform fixation	Image and sequence Onboard imaging, Direct in Sample Sequencing, & analysis	Secondary analysis Automated secondary pipelines	Visualize and interpret Multimodal visualization and downstream analysis

Flexible workflows to power your CRISPR screen

Choose the biological depth your screen needs, from optical phenotyping to full multimodal profiling.

	Guide 10s-10ks gRNA	Morphology 4-plex	Protein 20-plex	Transcriptome 3' poly-A
Cytos Optical Pooled Screening	✔️	✔️	✔️
Cytos Multimodal Pooled Screening	✔️	✔️	✔️	✔️

Get a full dataset

Explore the data from a multimodal pooled screen using Cytos on AVITI24.

Cytos Optical Pooled Screens (OPS) enables direct guide RNA sequencing and imaging of up to 24 morphological sturctures and protein markers with immunofluorescent based readouts. Cytos OPS uncovers insights into how perturbations shape organelle structures and protein localization and abundance for rich phenotyping.

Cytos Multimodal Pooled Screens (MPS) adds further biological resolution with unbiased in situ 3' transcriptome sequencing for the most comprehensive profiling of perturbation effects. Cytos MPS enables new discovery of pathway regulation, gene networks, and mechanistic insights.
No. Cytos Multimodal Pooled Screens enable the unique ability for high-resolution imaging of morphology and protein markers alongside full 3' transcriptome sequencing from a single cell.
With Cytos on AVITI24, you can capture guide RNA and transcriptome sequences directly onboard, removing any need for separate library preparation and Next Generation Sequencing (NGS) required by traditional workflows.
Cells are transduced, pooled, and plated on a Cytos Slide kit. After performing any treatments, cells are fixed and permeabilized, and the flow cell is assembled— all in a process that takes <1 hour. Imaging, sequencing, and key analysis steps are fully automated onboard, with configuration-dependent run times ranging from 24-90 hours. Using automated secondary analysis pipelines, data is ready to visualize the same-day.

Turn every perturbation into a multidimensional map

Link genetic perturbations to deep biological insight

More readouts, more of the picture

Cytos unlocks multimodal insights in one simple workflow

See the power of multimodal pooled screens with Cytos

Confidently assign perturbations into single cells

Link genetic perturbations to mophological phenotypes

Reveal transcriptome-wide effects of every perturbation

Visualize protein localization changes

From cells to answers

Flexible workflows to power your CRISPR screen

Get a full dataset

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FAQ

What can I measure in a CRISPR screen with Cytos?

Do I need to choose between imaging-based and transcriptome readouts?

Does CRISPR screening require library prep or NGS?

How much time does a CRISPR screen take with Cytos?

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Multimodal insights Validate each hit across morphology, protein, and transcriptomic response in the same cell.	Simple, integrated workflow Image, sequencing, and analyze onboard without separate library prep or external NGS.	Accelerate screen to mechanism Speed up screens with workflows as fast as 24 hours.