Element Elevate™ Library Prep Workflow

The Element Elevate Library Prep Workflow offers native library prep solutions for a wide range of applications, from whole-genome sequencing (WGS) and other DNA sequencing applications to RNA sequencing (RNA-Seq). Built on a foundation of efficient recovery and consistent results, the Elevate Workflow accommodates variable sample types and input amounts, including low input and low quality. Efficient, user-friendly protocols with onboard circularization minimize touchpoints and accelerate library prep time.

Elevate hero photo

Go End-to-End with Elevate

The Elevate Workflow features a modular kit design for greater flexibility. Mix and match library prep, enrichment, and index kits to configure your ideal end-to-end prep. Workflow choices include:

  • DNA library prep with or without PCR
  • Mechanical or enzymatic fragmentation of DNA samples
  • Messenger RNA (mRNA) capture or ribosomal RNA (rRNA) depletion for enrichment of RNA samples
  • Multiplexing of up to 96 samples with 96 unique dual indexes (UDIs) for accurate read assignment
  • Input optimizations for RNA integrity numbers (RINs) both above and below 7
  • Standalone index and adapter plates for custom library prep
  • Size selection that calibrates insert sizes

Elevate Specifications

Built to stringent specifications to support a variety of study requirements, DNA and RNA library prep kits prepare libraries for avidite base chemistry sequencing in less than four hours for DNA libraries and about six hours for RNA libraries. For complete compatibility information, see Product Compatibility.

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Specification

Mechanical Kit

Enzymatic Kit

RNA Kit

Kit size

24 reactions

24 reactions

24 reactions

Multiplexing

≤ 96 UDIs

≤ 96 UDIs

≤ 96 UDIs

Automation capability

Liquid handling robot

Liquid handling robot

Liquid handling robot

Input requirement

1–500 ng sheared genomic (gDNA)

1–500 ng gDNA

10–4000 ng RNA

Fragmentation method

Covaris shearing

Enzymatic

Enzymatic

Enrichment method

Not applicable

Not applicable

mRNA enrichment or rRNA depletion

PCR-free protocol time*

2 hours and 25 minutes

1 hour and 50 minutes

Not applicable

PCR-plus protocol time*

3 hours and 25 minutes

2 hours and 55 minutes

6 hours

Storage

-25°C to -15°C

-25°C to -15°C

-25°C to -15°C


* Approximate total time that includes hands-on time

Catalog Numbers

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Product

Catalog #

DNA Library Prep

Elevate Mechanical Library Prep Kit

830-00008

Elevate Enzymatic Library Prep Kit

830-00009

Elevate Long UDI Adapter Kit Set A

830-00010

Elevate Index and Adapter Kit
* This item will no longer be sold through Element Biosciences. Please order equivalent items from Integrated DNA Technologies (IDT) Catalog # 10017037 (96rxn size) or Catalog # 10017036 (16rxn size)

830-00005

RNA Library Prep

Elevate RNA Library Prep Kit

830-00019

Elevate mRNA Capture Kit for RNA Library Prep

830-00021

Elevate rRNA Depletion Kit for RNA Library Prep

830-00022

Elevate RNA Index and Adapter Kit

830-00020

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