Element Elevate™ Library Prep Workflow
The Element Elevate Library Prep Workflow prepares linear libraries for sequencing on the Element AVITI System. A long adapter design coupled with Cloudbreak™ sequencing chemistry eliminates manual circularization and enables the choice of a PCR-free or PCR-plus protocol.
The option of mechanical or enzymatic library prep further enhances flexibility and supports end-to-end DNA library prep. The ability to pair the standalone adapter kit with a third-party kit potentially expands applications and enables custom library prep. Either way, the result is efficient recovery and consistent results.
Elevate Workflow
Modular, mix-and-match kits to prepare linear libraries for sequencing on the AVITI System
Elevate Specifications
The Elevate Workflow supports three kits that supply reagents for mechanical or enzymatic library prep, adapters, and unique dual indexes (UDIs). The mechanical protocol starts with fragmented genomic DNA (gDNA). End repair and A-tailing keep the phosphate on the 5' end intact and ready the fragments for ligation, which adds adapters and UDIs. Starting with gDNA, the enzymatic protocol digests samples into fragments, then follows similar procedures.
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| Specification | Mechanical Kit | Enzymatic Kit | Adapter Kit |
|---|---|---|---|
Kit size | 24 reactions | 24 reactions | 96 reactions |
Multiplexing | ≤ 96 UDIs | ≤ 96 UDIs | ≤ 96 UDIs |
Automation capability | Liquid handling robot | Liquid handling robot | Depends on library prep kit |
Input requirement | 1–500 ng sheared gDNA | 1–500 ng gDNA | Not applicable |
PCR-free protocol time* | 2 hours and 25 minutes | 1 hour and 50 minutes | Not applicable |
PCR-plus protocol time* | 3 hours and 25 minutes | 2 hours and 55 minutes | Not applicable |
Fragmentation method | Covaris shearing | Enzymatic | Not applicable |
Storage | -25°C to -15°C | -25°C to -15°C | -25°C to -15°C |
System compatibility | AVITI System | AVITI System | AVITI System |
* Approximate total time that includes hands-on time
Catalog Numbers
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| Product | Catalog # |
|---|---|
Elevate Mechanical Library Prep Kit | 830-00008 |
Elevate Enzymatic Library Prep Kit | 830-00009 |
Elevate Long UDI Adapter Kit Set A | 830-00010 |
Cloudbreak PhiX Control Library, Elevate | 830-00017 |