The Element Adept Library Compatibility Workflow adapts linear libraries prepared with third-party kits for sequencing on the Element AVITI™ System. Leveraging a custom splint mix that adds Element surface primers to compatible libraries, this workflow grants the benefit of adapting a wide variety of existing libraries without changing your upstream library prep. This approach unlocks access to a wide variety of applications, including whole-genome sequencing (WGS), RNA sequencing (RNA-Seq), single-cell assays, and beyond. Visit Compatible Kits for the Element Adept Library Compatibility Workflow for an up-to-date list of compatible third-party kits.
Adept Library Compatibility Workflow
Simple circularization of linear libraries for Sequencing by AVITI
The Adept Workflow is a one-kit workflow comprised of the Element Adept Library Compatibility Kit, which includes familiar reagents and procedures to circularize libraries for sequencing in only 75 minutes. The protocol starts with 0.5 pmol of linear library that is annealed to splint oligos, adding Element surface primers. A ligation reaction then circularizes the library, followed by a digestion reaction to remove any leftover splint oligos or linear library material. A final bead cleanup removes small materials, salts, and enzymes.
|Kit configuration||Element Adept Library Compatibility Kit, 24 reactions (catalog # 830-00003)|
|Storage temperature||-25°C to -15°C|
|Input requirement||0.5 pmol linear library per reaction|
~75 minutes total time~20 minutes hands-on time
|Indexing||≤ 10 bp dual indexes and unique molecular identifier (UMI) support|
|Sequencing||Element AVITI System|
Optional PhiX Control Library, Adept (catalog # 830-00004)