AVITI24 System and Specifications

    One integrated platform for single cell multiomics and sequencing

    Multiomics

    RNA, protein, & morphology profiled simultaneously

    24 hr

    Run times

    2M cells

    Profiled per run at subcellular resolution

    20 cm2

    Imaging area per run

    aviti24-platform-figures-03

    Flexible

    2 independent flow cells with
    2 individually addressable lanes

    Q50

    Sequencing read quality Cloudbreak UltraQ

    3B reads

    Run output for 2 x 150

    <$1

    Per million reads

    Powered by the flexibility of ABC sequencing, AVITI24 is the first and only platform to enable co-detection of RNA, protein, and morphology and NGS in one dual-sided run. With a first-of-its-kind onboard image processing system, AVITI24 seamlessly delivers industry-leading data quality. Independent run starts and ultra-fast run times provide unparalleled flexibility to accelerate your discovery.

    Specifications

    Sequencing Specifications

    Read Counta

    High output:
    Medium output:
    Low output:

    1.5 B reads per flow cell
    750 M reads per flow cell
    375 M reads per flow cell

    Accuracy

    > 90% Q30 with 2 x 150 and 2 x 75 cycles
    > 85% Q30 with 2 x 300 cycles
    > 70% Q50 with Cloudbreak UltraQ™ kitsc

    Inputs

    Direct loading of linear libraries with Cloudbreak Freestyle™ kits

    Run Timeb

    ~24 hours 2 x 75 cycles
    ~38 hours 2 x 150 cycles
    ~60 hours 2 x 300 cycles

     

    a Performance metrics are based on sequencing Element 2x150 libraries. Actual results might differ due to library type and preparation methods. Increased sequencing output available on Cloudbreak and Cloudbreak Freestyle 2 x 75 and 2 x1 50 sequencing kits at this time.

    b Individually addressable lanes and custom recipes can extend run times.

    c Based on Elevate™ libraries and specific run parameters.

    Cytoprofiling Specifications

    Analytes

    RNA, protein, morphology
    100 bp in situ RNA sequencing in 2025

    Plex

    RNA: 350 targets
    Protein: 50 targets
    Morphology: 6 markers

    Content

    MAP Kinase Cell Cycle and Apoptosis
    Immunology, neuroscience, and custom panels in 2025

    Imaging

    < 250 nm subcellular spatial resolution with multi-feature cell segmentation

    Inputs

    Adherent cells
    Cell suspensions in 2025

    Sensitivity

    1 M mean counts detected per mm²

    Throughput

    Up to 1 M cells with 10 cm² area per flow cell
    Two flow cells per run

    Format

    12 wells (0.5 cm²/well)
    1 well (10 cm²/well)

    Run Time

    24 hoursa

    Sample Prep

    45 minutes

    Run times based on a single 12 well run with software update available in early 2025.

    System Specifications

    Instrument Configuration

    Dual flow cells
    AVITI Operating Software
    Ubuntu Core 20.04 LTS operating system

    Operating Environment

    Temperature: 18°C to 26°C
    Elevation: < 2000 m
    Sound level: ≤ 62 db at 3.3 ft

    Instrument Dimensions

    (H x W x D) 29.5 in x 37.6 in x 28.5 in
    Weight: 155.1 kg/342 lb

    Power Requirements

    100–240 VAC at 50/60 Hz
    15 A, 550 W (average)

    Ordering Information

    Element AVITI24 System

    880-00004

    Element AVITI24 Upgrade

    895-00060

    Start your AVITI24 journey

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