Resolve the biology behind drug response

	Hit confirmation Confirm phenotypes before advancing into lead optimization.		On- vs. off-target activity Distinguish on-target effects from off-target and non-specific stress responses.
	Mechanism of action Resolve the molecular pathways and protein activity driving a compound's phenotypic effect.		Toxicity screening Characterize toxicity signatures across doses, timepoints, and cell states.
	Lead optimization Rank and advance compound series by potency, selectivity, and phenotype in parallel.		Heterogenous response resolution Identify rare responders and cell-state-specific effects.

Drug response is multidimensional. Today's assays capture only part of the picture.

Morphology, protein, and transcriptomic assays each reveal a different dimension of how cells respond to treatment. Connecting these separate measurements is critical to build a more complete picture of efficacy, mechanism, and toxicity.

Cytos connects every dimension of drug response

Bring morphology, protein, and transcriptomics together in a single integrated workflow to connect cellular phenotype with the mechanisms driving response. Generate deeper insights from every screen to qualify leads with greater confidence, while replacing multiple disconnected assays with one streamlined experiment.

Same-cell, multimodal profiling Capture morphology, transcriptome and protein data from the same cells instead of piecing together results from separate assays.	Link phenotype to mechanism Connect a visible phenotypic change to the gene programs and pathways driving it, so biology can be explained, not just observed.	Accelerate time to insight Replace separate assays, sample preps, with a single automated workflow, cutting the time and complexity of your experiments.

Explore multimodal drug screening with Cytos

	EXPERIMENTAL DESIGN Profile up to 96 conditions in a dual-sided run Explore compound, dose, and time-dependent responses across up to 96 sections in a dual-sided run. Capture morphology, protein, and RNA together to generate a multidimensional view of drug response without running separate assays.
	MORPHOLOGY Rapidly identify compounds that drive a phenotypic response Quantify treatment-induced changes in cell morphology to distinguish active compounds and characterize dose-dependent responses. Resolve heterogenous responses at single-cell resolution to identify phenotypes that population-level measurements may miss.
	3' TRANSCRIPTOME Discover the pathways driving drug response Sequencing the 3' transcriptome directly in cells to reveal pathways and cellular programs altered by treatment. Discover mechanisms, stress responses, and potential off-target effects without separate library preparation.
	PROTEIN Measure protein activity where it happens Measure treatment-induced changes in protein abundance, activation, and localization at single-cell resolution. Confirm pathway engagement and connect molecular changes to phenotypes observed in your screen.

From compounds to conclusions

Drug or compounds treatment Screen up to 96 samples across doses, timepoints, and compounds	Culture and fix cells Culture cells directly on AVITI24™ slides & perform fixation	Image and sequence Onboard imaging, Direct in Sample Sequencing, & analysis	Secondary analysis Automated secondary pipelines	Visualize and interpret Multimodal visualization and downstream analysis