The traditional hybrid selection process for targeted panels, like exomes, involves a time-consuming process with multiple temperature-controlled washes, PCR-based amplification, and library quality control.

With the simplified Trinity workflow, you simply hybridize your DNA library to the target probes of interest, wait for the prescribed time, and load it directly onto an AVITI—drastically reducing manual steps and additional amplification resulting in higher library complexity and lower duplication rates.

Trinity not only improves the workflow of hybrid selection sequencing, it also improves performance with a dramatic reduction in duplication rate and higher library complexity when compared to libraries run in-solution. This allows for more efficient sequencing where we see higher coverage per sequencing read.

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Trinity sequencing kits are optimized for sequencing 24 exomes per flow cell on AVITI, or 36 exomes on the AVITI24™ system, achieving ≥ 50x mean target coverage, and the ability to scale the plexity depending on your coverage needs. For added TAT flexibility, Trinity is available in both 2 x 75 and 2 x 150 options.