Element Adept™ Library Compatibility Workflow

    The Adept Workflow makes libraries prepared with third-party kits compatible with the Element AVITI™ System without changes to upstream library prep or analysis. Simply augment your existing sequencing workflow with AVITI to capitalize on cost, flexibility, and quality for any project.

    Custom splint oligos kitted with circularization or amplification reagents unlock a diversity of applications, from exomes to whole-genome sequencing (WGS). Multiple kit and protocol options ensure library compatibility and choice of key experiment parameters.

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    Adept Library Compatibility Workflow

    Protocol Element Kit Compatible Input Final Library Circularization Method Quantification Method

    Adept Standard

    Element Adept Library Compatibility Kit v1.1

    Reach out to Element Tech Support for a full list

    Circular On-bench qPCR

    Adept Rapid PCR-Free

    Element Adept Library Compatibility Kit v1.1

    Reach out to Element Tech Support for a full list Circular On-bench qPCR

    Adept Rapid PCR-Plus

    Adept Rapid PCR-Plus Kit

    Any library Linear Onboard Qubit

    The Element Adept Library Compatibility Kit v1.1 (Catalog #830-00007) offers benchtop circularization of libraries prior to sequencing. We have verified compatibility of this kit with many 3rd party sequencing assays. For complete circularization, all assays require full-length libraires without 5’ or 3’ additions, truncations, or modifications. Please reach out to your Field Applications Scientist or Element Tech Support for more information.

    Kit Specifications

    The Element Adept Library Compatibility Kit v1.1 and Adept Rapid PCR-Plus supply familiar reagents and procedures that are complete in as little as 40 minutes. Depending on protocol, you can manually circularize libraries on-bench or amplify the libraries for automatic circularization onboard the instrument as part of the run.

    Specification Adept Library Compatibility Kit v1.1 Adept Rapid PCR-Plus Kit

    Kit size

    24 reactions

    24 reactions

    Indexing

    ≤ 10 bp dual index

    ≤ 10 bp dual index

    UMIs

    Supported

    Supported
    Storage -25°C to -15°C -25°C to -15°C
    Sequencing AVITI AVITI
    Adept Standard Protocol    
    Input/reaction 0.2–0.5 pmol Not applicable
    Total time ~75 minutes Not applicable
    Hands-on time ~25 minutes Not applicable
    Adept PCR-Free Protocol    
    Input/reaction 0.1–0.5 pmol Not applicable
    Total time ~40 minutes Not applicable
    Hands-on time ~10 minutes Not applicable
    Adept PCR-Plus Protocol    
    Input/reaction Not applicable 0.05 pmol
    Total time Not applicable ~40 minutes
    Hands-on time Not applicable ~20 minutes

    Catalog Numbers

    Product Catalog #

    Element Adept Library Compatibility Kit v1.1

    830-00007

    Adept Rapid PCR-Plus Kit

    830-00018