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      Element Elevate™ Library Prep Workflow

      The Element Elevate Library Prep Workflow offers native library prep solutions for a wide range of applications including whole-genome sequencing (WGS) and other DNA sequencing applications. Built on a foundation of efficient recovery and consistent results, the Elevate Workflow accommodates variable sample types and input amounts, including low input and low quality. Efficient, user-friendly protocols with onboard circularization minimize touchpoints and accelerate library prep time.

      See the DNA Specification Sheet
      elevate-hero-photo
      See the DNA Specification Sheet

      Go End-to-End with Elevate

      The Elevate Workflow features a modular kit design for greater flexibility. Mix and match library prep, enrichment, and index kits to configure your ideal end-to-end prep. Workflow choices include:

      • DNA library prep with or without PCR
      • Mechanical or enzymatic fragmentation of DNA samples
      • Multiplexing of up to 96 samples with 96 unique dual indexes (UDIs) for accurate read assignment
      • Standalone index and adapter plates for custom library prep
      • Size selection that calibrates insert sizes

      Elevate Specifications

      Built to stringent specifications to support a variety of study requirements, DNA library prep kits prepare libraries for avidite base chemistry sequencing in less than four hours. For complete compatibility information, see Product Compatibility.

      Specification

      Mechanical Kit

      Enzymatic Kit

      Kit size

      24 reactions

      24 reactions

      Multiplexing

      ≤ 96 UDIs

      ≤ 96 UDIs

      Automation capability

      Liquid handling robot

      Liquid handling robot

      Input requirement

      1–500 ng sheared genomic (gDNA)

      1–500 ng gDNA

      Fragmentation method

      Covaris shearing

      Enzymatic

      Enrichment method

      Not applicable

      Not applicable

      PCR-free protocol time*

      2 hours and 25 minutes

      1 hour and 50 minutes

      PCR-plus protocol time*

      3 hours and 25 minutes

      2 hours and 55 minutes

      Storage

      -25°C to -15°C

      -25°C to -15°C

       

      * Approximate total time that includes hands-on time

      Catalog Numbers

      Product

      Catalog #

      DNA Library Prep

      Elevate Mechanical Library Prep Kit

      830-00008

      Elevate Enzymatic Library Prep Kit

      830-00009

      Elevate Long UDI Adapter Kit Set A

      830-00010

      Elevate Index and Adapter Kit
      * This item will no longer be sold through Element Biosciences. Please order equivalent items from Integrated DNA Technologies (IDT) Catalog # 10017037 (96rxn size) or Catalog # 10017036 (16rxn size) or from Twist Biosciences Catalog # 107682 (96 samples)

      830-00005

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