Hybrid capture technology is a powerful and cost-effective tool to detect somatic mutations in n precision oncology research. While target capture is important for efficient mutation detection, obtaining rapid sequencing results is critical. Traditional hybrid capture workflows involve several time-consuming steps prior to sequencing which can delay results.
Here, we combine Trinity™ hybrid capture and a rapid AVITI™ sequencing protocol to deliver targeted sequencing results within 12 hours. We applied this specialized Trinity workflow with a custom myeloid panel based on a design used by Genomics Medicine Sweden to detect mutations in reference DNA and patient blood/bone marrow/fresh frozen tissue. This rapid, accurate, and robust solution for targeted sequencing, offers significant advancements in turnaround time for hematology malignancy research.