Quick Start Guides
Cloudbreak Sequencing Quick Guide (MA-00009 Rev L)
Element Elevate Library Prep Quick Guide for the Enzymatic Protocol (MA-00038)
Element Elevate Library Prep Quick Guide for the Mechanical Protocol (MA-00005)
Element Adept Library Compatibility Workflow Quick Guide for the Standard Protocol (MA-00002)
Element Adept Library Compatibility Workflow Quick Guide for the Rapid PCR-Plus Protocol (MA-00041)
Element Adept Library Compatibility Workflow Quick Guide for the Rapid PCR-Free Protocol (MA-00034)
Twist Fast Hyb & Trinity Freestyle Run Setup Quick Guide (MA-00079)
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User Guides
Cloudbreak Sequencing User Guide (MA-00058 Rev H)
Technical Notes
Enhanced Output with AVITI24™ System (LT-00061)
Expert Mode HD Tech Note (LT-00039)
Sequencing with Cloudbreak and Cloudbreak Freestyle Technical Note (LT-00026)
Element Biosciences Library Sequences Technical Note (LT-00014)
Knowledge Base Articles
How should I prepare my long insert libraries for Cloudbreak Freestyle Sequencing?
How should I prepare my libraries for Cloudbreak UltraQ Sequencing?
Can I use custom primers on the AVITI?
Are HPLC-purified indexing primers required for library preparation when using Cloudbreak Freestyle for sequencing?
What are some best practices for library quantification and loading to maximize my sequencing run density?
How much library should I load with AVITI24 to maximize output?
Are Elevate, Adept, and Freestyle 3rd Party PhiX interchangeable?
Assessing Your Cloudbreak and Cloudbreak Freestyle Sequencing Runs
What are pass-filter reads and how do I calculate pass-filter rate?
Guidance for discarding a run setup after a flow cell is loaded and instrument priming is complete
Does the QIAseq miRNA Library Kit require a custom sequencing primer on AVITI?
How can library pools with samples of varying index lengths be sequenced on the AVITI and demultiplexed in Bases2Fastq?
Can libraries that have undergone Adept conversion be sequenced with Cloudbreak Freestyle sequencing reagent kits?
Can I set up a run without a run manifest?
Where can I see examples of underloaded and overloaded thumbnail images?
Which sequencing recipe should I use for my library insert size?
When should I enable Expert Mode HD for my sequencing run?
How does increased sequencing output on AVITI24‚Ñ¢ compare to performance on AVITI with and without Expert Mode HD?
Will I still experience output improvements on AVITI24 without increasing library loading concentration?
Can I use asymmetric read length conditions on a run, i.e. 200+100 for a 300 cycle kit?
What is the maximum allowable index length?
Can we run different types of libraries on the flow cell lanes?
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Software Documentation
Getting started with Bases2Fastq for sequencing
Prepare a sequencing manifest