Cell Culture Protocols for use with Cytos™ Cell Screening

    Reference protocols for cell culture, organized.

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    Validated
    Validated Protocols
    These protocols have been validated across numerous runs using Cytos kits for cell screening on the AVITI24™ system, and include detailed cell culture protocols with seeding densities and images showing successful cell culture techniques.
    JurkatDownload
    SH-SY5YDownload
    Demonstrated
    Demonstrated Protocols
    These protocols may include customer or internal protocols that have been tested with Cytos kits for cell screening on a limited number of runs and may require additional optimization.
    iPSC-derived neuronLink pending
    iPSC-derived microgliaLink pending
    Fibroblasts (IMR-90, BJ)Link pending
    Primary hepatocytesLink pending
    Note: These protocols are designed for wild-type, untreated cell lines. Treatment, handling, cell line differences, passage number, and other factors can affect culture behavior, so some optimization is typically expected. See Cytos Sample Preparation & Optimization User Guide for more specific guidance.