Poster: Single-cell multiomics to capture consequences of acutely depleting the neurodevelopmental gene SETD5 in hiPSCs

Steady-state perturbations blur primary regulatory events together with downstream cascades. See how a time-series of single-cell multiomics captures the transcriptional and morphological consequences of acutely depleting SETD5, a neurodevelopmental gene, in hiPSCs. 

What you’ll learn:

• How a single AVITI24™ run measured protein, whole-transcriptome 3’ RNA, and 240 morphology features per cell in hiPSCs with acute SETD5 depletion. 
• How we identified differentially expressed genes, including transcriptional programs that bulk aggregation masked until cells were conditioned on morphology-defined state.
• How the study extends to a time course to separates immediate-early responses from sustained or delayed ones.