Poster: Next generation multiplex hybrid capture and sequencing

Hybrid capture delivers deep, uniform coverage, but the workflow is complex and high-plex pooling can reduce library complexity diminishing sensitivity for low frequency variants. Here, we present a streamlined workflow using Trinity™ sequencing, paired with a new hybridization buffer that preserves library complexity. 

What you’ll learn:

• How on-flow-cell capture and washing eliminate post-hybridization processing and post-hybridization PCR.
• How a novel hybridization buffer maintains high library complexity and uniformity with 24-sample multiplexing.
• Robust performance across exome and comprehensive genomic profiling (CGP) panels, including 87-88% on-target bases and 52-56x mean target coverage.
• How this workflow exhibited low duplication rates and high library complexity for sensitive variant detection.