2 x 300 Long-Insert Human WGS with the Elevate™ Workflow

Cloudbreak chemistry performed long-insert whole-genome sequencing (WGS) of a human reference genome prepared with the Element Elevate Library Prep Workflow. 

Methods 

PCR-free libraries were prepared from 1 µg HG001. The samples were mechanically sheared to ~800 bp inserts, end-repaired, and ligated with Elevate adapters. The Elevate libraries were denatured and loaded as single stranded linear DNA fragments and sequenced on the AVITI™ System using a 2 x 300 read length. Bases2Fastq generated the FASTQ files.