Human Whole-Genome Sequencing, Third Party (Cloudbreak Freestyle™)

Cloudbreak Freestyle chemistry was used to perform whole-genome sequencing (WGS) of human reference genomes. 

Methods 

Four total PCR-free libraries were prepared from input of 1 µg of HG001 and HG002 gDNA using the KAPA HyperPlus Kit. The samples were mechanically sheared to two different insert sizes (~400 bp and ~500 bp), end-repaired, and ligated with KAPA unique dual index (UDI) adapters. Libraries were sequenced using Cloudbreak Freestyle chemistry on the AVITI™ System with a 2 x 150 read length. Bases2Fastq was used to generate the FASTQ files.