Human Whole-Genome Sequencing (Cloudbreak™)

Cloudbreak chemistry performed whole-genome sequencing (WGS) of human reference genomes.

Methods 

PCR-free libraries were prepared from 1 µg HG001, HG002, HG003, and HG004. The samples were mechanically sheared to ~500 bp or ~1000 bp inserts, end-repaired, and ligated with KAPA unique dual index (UDI) adapters. The Adept™ Library Compatibility Kit v1.1 circularized the libraries for sequencing on the AVITI™ System using a 2 x 150 read length with a custom recipe for 1000 bp inserts. Bases2Fastq generated the FASTQ files.