Human Whole-Exome Sequencing, xGen™ Hyb Capture Kit Trinity for Element (Trinity™)

The Trinity Sequencing Kit was used to perform whole-exome sequencing (WES) on reference genomic DNA (gDNA).

Methods

IDT xGen Exome Sequencing Kit Trinity for Element (#10022463) was used to prepare libraries and perform capture for Trinity sequencing according to the user guide.

Libraries were prepared from 100 ng of NA12878 gDNA, with 7 PCR cycles performed for amplification and indexing of the replicates. A total of 24 libraries were pooled equally by mass (250 ng per sample) in a single overnight hybridization reaction.

Following capture, the libraries were sequenced using the Trinity 2x150 Sequencing Kit (#860-00020). Bases2Fastq was used to generate adapter trimmed FASTQ files from the sequencing data.