Human Whole-Exome Sequencing, Twist for Element Trinity, Exome 2.0 + Comp Spike, Fast Hyb Workflow, 2 x 75 sequencing (Trinity™ Sequencing Kit)

The Trinity sequencing kit was used to perform whole-exome sequencing (WES) on reference genomic DNA (gDNA).

Methods

The Twist for Element Trinity, Exome 2.0 + Comp Spike, Fast Hyb Workflow (#109327) was used to prepare libraries and perform capture for Trinity sequencing according to the user guide.

Libraries were prepared from 50 ng of NA12878 gDNA, with 8 PCR cycles performed for amplification and indexing of the replicates. A total of 8 libraries were pooled equally by mass (500 ng per sample) per 1 hr fast hybridization capture reaction. Three total hybridization reactions were prepared.

Following capture, the three hybridization reactions were pooled and sequenced using the Trinity 2x75 Sequencing Kit (#860-00019). Bases2Fastq was used to generate FASTQ files from the sequencing data.