2 x 300 16S Amplicon Sequencing

Cloudbreak chemistry was used to perform amplicon sequencing of the 16S ribosomal (rRNA) gene.

Methods 

The V3 and V4 regions of bacterial 16S rRNA genes were PCR-amplified from a ZymoBIOMICS Microbial Community DNA Standard using KAPA HiFi HotStart ReadyMix. Adept libraries were pooled as a 64-plex and sequenced on the AVITI™ System using a 2 x 300 read length. Bases2Fastq generated the FASTQ files.