Protein Overview
ATP1A1 encodes the catalytic alpha-1 subunit of the Na⁺/K⁺-ATPase, a P-type ATPase that hydrolyzes ATP to pump Na⁺ out of and K⁺ into the cell, establishing the electrochemical gradients essential for membrane potential, secondary active transport, and cell volume regulation. It also participates in cell-cell adhesion and intracellular signaling (e.g., Src kinase interactions).
Expected Localization
Plasma membrane protein; in polarized epithelial cells it localizes to the basolateral membrane domain (excluded from the apical surface), while in neurons/cardiomyocytes it distributes broadly across the membrane.
Immunofluorescence shows a sharp, continuous membrane-outline staining pattern.
Expected Expression Pattern
Ubiquitously expressed alpha-1 isoform found in essentially all tissues, with especially high levels in kidney, brain, heart, and skeletal muscle. Expression can vary with polarization state, differentiation, and hormonal/ionic regulation (e.g., aldosterone, thyroid hormone).
Relevant Pathways
- Ion homeostasis and membrane potential maintenance
- Epithelial transcellular transport and osmoregulation
- Cardiac/neuronal action potential generation and excitability
- Secondary active transport (Na⁺-coupled cotransport)
- Cell-cell adhesion and cytoskeletal anchoring at the basolateral membrane
Clone Information
Antibody Species: Rabbit
Cell Type Compatibility: Validated in human cells only using Cytos™ kits.
For more details on the clone and reactivity with other species, visit ABclonal and search for the clone catalog # A11683BCS.
Qualitative Results
Localization Assessment
Na⁺/K⁺ ATPase protein panel demonstrates strong localization to the cell membrane. The observed localization of Na⁺/K⁺ ATPase protein is consistent with published data.
HeLa |
HeLa |
HeLa |
HeLa |
HeLa cells were cultured in a 12-well format Cytos slide kit overnight then fixed with 4% formaldehyde for 30 minutes, permeabilized using 70% ethanol for 10 minutes. The slide kit was then assembled into a flow cell and loaded onto an AVITI24™ along with the Cytos Protein Cartridge for a multiomics cell screening run. Na⁺/K⁺ ATPase demonstrated cell membrane localization and high signal-to-noise ratio, with no detectable probe crosstalk. These results support high confidence in target-specific detection and validate this antibody as a passing clone. A549 cells were also cultured and processed under the same protocol in parallel as part of Cytos cross-run reproducibility testing.
Quantitative Results
| Signal-to-noise ratio Acceptance criteria: > 2x background |
Pass |
- 10.3 ± 2.5 (HeLa; n=26 runs)
- 7.6 ± 1.1 (A549; n=34 runs)
Indicates the fold brightness of the target signal as measured by the Mean Intensity Above (Otsu-defined) Threshold (IAT), over the background (a channel-matched isotype control) in the same cell. Data shown are mean ± standard deviation across independent reproducibility runs. The signal is consistently over 3 times above the acceptance threshold in both HeLa and A549 cells — a clean, easily distinguishable signal.
|
Panel-level run-to-run reproducibility |
Pass |
For each imaging panel, we assess reproducibility by comparing the intensity signal a given antibody target produces across independent replicate runs. Within each run, we measure the average per-well fluorescence intensity for every target in the panel. We then plot each target's intensity from one run against the matching target's intensity from another run of the same layout, and calculate the Pearson correlation coefficient (r) between those two sets of measurements. Squaring that value gives the R² (coefficient of determination), which reports what fraction of the variation in one run's signal is explained by the other run's signal.
Disclaimer: This information is provided to help assess whether the target suits your experiment. It reflects internal testing conditions and does not guarantee performance in your application. Performance in other cell types and species such as mouse or rat may require additional validation using Cytos kits as performance is not guaranteed.
ELEMENT BIOSCIENCES For Research Use Only. Not for Use in diagnostic procedures.