Protein Overview
GM130, encoded by GOLGA2, is a cis-Golgi matrix protein that contributes to Golgi ribbon organization, vesicle tethering, and directional trafficking between the endoplasmic reticulum and Golgi apparatus. GM130 also participates in Golgi disassembly and reassembly during mitosis and helps coordinate cell polarity and centrosome-related processes.
Expected Localization
Cis-Golgi apparatus.
In interphase cells, GM130 typically appears as a compact, ribbon-like or clustered perinuclear structure positioned adjacent to the nucleus. The Golgi pattern becomes dispersed or fragmented during mitosis and after Golgi-disrupting treatments.
Expected Expression Pattern
Broadly expressed in nucleated mammalian cells with an intact Golgi apparatus. Staining morphology may vary with cell shape, secretory activity, cell-cycle stage, and treatments that alter Golgi organization.
Relevant Pathways
- Golgi organization and maintenance
- ER-to-Golgi vesicle trafficking
- Protein processing and secretion
- Cell polarity and migration
- Mitotic Golgi disassembly and reassembly
Clone Information
Antibody Species: Rabbit
Cell Type Compatibility: Validated in human cells only using Cytos™ kits.
For more details on the clone and reactivity with other species, visit Novus and search for the clone catalog # NBP2-53420B .
Qualitative Results
Localization Assessment
GM130 protein panel demonstrates strong localization to the Golgi. The observed localization of GM130 protein is consistent with published data.
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| HeLa | HeLa |
HeLa cells were cultured in a 12-well format Cytos slide kit overnight then fixed with 4% formaldehyde for 30 minutes, permeabilized using 70% ethanol for 10 minutes. The slide kit was then assembled into a flow cell and loaded onto an AVITI24™ along with the Cytos Protein Cartridge for a multiomics cell screening run. GM130 demonstrated strong Golgi localization and high signal-to-noise ratio, with no detectable probe crosstalk. These results support high confidence in target-specific detection and validate this antibody as a passing clone. A549 cells were also cultured and processed under the same protocol in parallel as part of Cytos cross-run reproducibility testing.
Quantitative Results
| Signal-to-noise ratio Acceptance criteria: > 2x background |
Pass |
- 6.3 ± 2.2 (HeLa; n=26 runs)
- 6.6 ± 2.5 (A549; n=34 runs)
Indicates the fold brightness of the target signal as measured by the Mean Intensity Above (Otsu-defined) Threshold (IAT), over the background (a channel-matched isotype control) in the same cell. Data shown are mean ± standard deviation across independent reproducibility runs. The ratio ranges from 6.3–6.6 across HeLa and A549 cells, consistently over 3 times the acceptance threshold — a clean, easily distinguishable signal, together with the high specificity already demonstrated by the localization assessment.
|
Panel-level run-to-run reproducibility |
Pass |
For each imaging panel, we assess reproducibility by comparing the intensity signal a given antibody target produces across independent replicate runs. Within each run, we measure the average per-well fluorescence intensity for every target in the panel. We then plot each target's intensity from one run against the matching target's intensity from another run of the same layout, and calculate the Pearson correlation coefficient (r) between those two sets of measurements. Squaring that value gives the R² (coefficient of determination), which reports what fraction of the variation in one run's signal is explained by the other run's signal.
Disclaimer: This information is provided to help assess whether the target suits your experiment. It reflects internal testing conditions and does not guarantee performance in your application. Performance in other cell types and species such as mouse or rat may require additional validation using Cytos kits as performance is not guaranteed.
ELEMENT BIOSCIENCES For Research Use Only. Not for Use in diagnostic procedures.