Compatible Kits for the Element Adept™ Library Compatibility Workflow

The Element Adept™ Library Compatibility Workflow adapts linear libraries prepared with third-party kits for sequencing on the Element AVITI™ System. With a simple workflow and only ~75 minutes of total protocol time, an Adept reaction can process up to 384 pooled libraries and generate sequencing-ready circular libraries.

The Adept Workflow supports libraries that match the sequence Figure 1 depicts in black. Libraries with missing, additional, or mismatched bases or 5′ and 3′ modifications that block DNA ligation require amplification before the protocol starts. The Element Adept Library Compatibility Workflow Guide (MA-00001) provides instructions.

Figure 1
Figure 1. Compatible library

Compatible Assays

The following table lists library prep and index kits that have been successfully processed and demonstrate compatibility with the Adept Workflow. These compatible assays generate libraries that are ready input for the Adept Workflow.

The Element Adept Library Compatibility Kit v1.1 updates a circularization reagent and uses qPCR Primer Mix 2 to quantify libraries in both N and T categories and libraries with custom sequencing adapters. Thus, assays that were incompatible with the Element Adept Library Compatibility Kit are compatible with Adept v1.1.

CategoryVendorWorkflowExample Catalog #AdeptAdept v1.1
N10x GenomicsChromium Next GEM Single Cell ATAC Library & Gel Bead Kit, 16 rxns1000175YesYes
N10x GenomicsSingle Index Kit N Set A1000212YesYes
T10x GenomicsChromium Next GEM Single Cell 3′ GEM, Library & Gel Bead Kit v3.1, 16 rxns1000121YesYes
T10x GenomicsChromium Next GEM Single Cell 5′ Library & Gel Bead Kit v1.1, 16 rxns1000165YesYes
T10x GenomicsChromium Single Cell V(D)J Enrichment Kit, Human B Cell, 96 rxns1000016YesYes
T10x GenomicsVisium Spatial for FFPE Gene Expression Kit, Mouse Transcriptome, 4 rxn1000339YesYes
T10x GenomicsSingle Index Kit T Set A1000213YesYes
T10x GenomicsDual Index Kit TT, Set A1000215YesYes
N10x GenomicsChromium Next GEM Single Cell ATAC Kit v2, 16 rxns1000390YesYes
N10x GenomicsDual Index Kit NN Set A (for Sample Multiplexing)1000243YesYes
T10x GenomicsVisium Spatial Gene Expression Slide & Reagent Kit1000184YesYes
TAgilentSureSelect XT-LI with OneSeq v65190-8863YesYes
TAgilentSureSelect XT HS v2 with Human Exome v85191-6873YesYes
TAgilentAgilent SureSelectXT Human Methyl-Seq5190-4662, G9651BNoYes*
TAgilentSURESELECT XT HS2 Reagent Kit with Index Primer PairsG9983A (pairs 1–96)YesYes
TAgilentSURESELECT XT HS2 Reagent Kit with Index Primer PairsG9983B (pairs 97–192)YesYes
TAgilentSURESELECT XT HS2 Reagent Kit with Index Primer PairsG9983C (pairs 193–288)YesYes
TAgilentSURESELECT XT HS2 Reagent Kit with Index Primer Pairs**G9983D (pairs 289–384)YesYes
TAgilentSSELEXT Human All Exon V8, 16 Rxns5191-6879YesYes
TBD BiosciencesWhole Transcriptome Analysis (WTA) Amplification Kit633801YesYes
CBio-RadSureCell ATAC-Seq Library Prep Kit17004620NoYes
TBio-RadSEQuoia Express Stranded RNA Library Prep Kit12017265YesYes
TDovetail GenomicsDovetail Micro-C Kit21006YesYes
TDovetail GenomicsDovetail Omni-C Kit21005YesYes
TIDTxGen Exome Research Panel v210005151YesYes
TIDTIDT xGen EZ library prep10009821YesYes
TIDTxGen cfDNA & FFPE DNA Library Prep Kit10006203YesYes
TIDTxGen DNA Lib Prep EZ 16rxn10009863YesYes
TIDTxGen Stubby Adapter and Unique Dual Index Primer Pairs, 96 rxn10005921YesYes
TIDTxGen SARS-CoV-2 amplicon panels10009832YesYes
TIDTxGen Methyl-Seq Library Preparation (Swift Biosciences Accel-NGS Methyl-Seq DNA library Kit)10009824NoYes*
CIlluminaTruSeq Small RNA Library Prep KitRS-200-0012NoYes
NIlluminaNextera XT DNA Library Preparation Kit (24 Samples)**FC-131-1024YesYes
NIlluminaIllumina DNA Prep, (M) Tagmentation (24 Samples)20018704YesYes
TIlluminaTruSeq Stranded Total RNA Prep Kit20020596NoYes*
TJumpcodeCRISPRclean Plus Stranded Total RNA Prep with rRNA Depletion (Human, Mouse, Rat, Pan Bacteria)KIT1016YesYes
TLexogenQuantSeq 3′ mRNA-Seq Library Prep Kit FWD15.96YesYes
TNew England BiolabsNEBNext Multiplex Oligos for Illumina (Methylated Adaptor, Index Primers Set 1)E7535NoYes*
TNew England BiolabsNEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 2)E6442S, E6442LNoYes*
TNew England BiolabsNEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors DNA Set 1)E7395SYesYes
TNew England BiolabsNEBNext Ultra II Directional RNA Library Prep Kit for Illumina, 24 reactionsE7760SYesYes
TNew England BiolabsNEBNext Ultra II FS DNA Library Prep Kit for Illumina, 96 reactionsE7805LYesYes
TNew England BiolabsNEBNext Ultra II DNA Library Prep Kit for IlluminaE7645LYesYes
TNew England BiolabsNEBNext Ultra II DNA Library Prep Kit for Illumina with Sample Purification BeadsE7103LYesYes
TNew England BiolabsNEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors)E7395SYesYes
TParse BiosciencesEvercode Whole Transcriptome8098115702YesYes
TPerkinElmerNEXTFLEX Variant-Seq SARS-CoV-2 V2 KitNOVA-5350-096YesYes
TPerkinElmerNEXTFLEX Rapid XP V2 DNA-seq kitNOVA-5149-01,02,03,103YesYes
TPerkinElmerNEXTFLEX Unique Dual Index Barcodes - Set ANOVA-514150,1,2,3YesYes
CQIAGENQIAseq Multimodal Panel333932NoYes*
TQIAGENQIAseq FX DNA Library Kit180479YesYes
TQIAGENQIAseq miRNA Library Kit331505YesYes
TRocheKAPA mRNA HyperPrep Kit, 24 libraries8098115702YesYes
TRocheKAPA HyperPlus Kit With Library Amplification, 96 reactions7962428001YesYes
TRocheKAPA HyperPrep Kit without amplification module, 96 reactions7962371001YesYes
TRocheKAPA EvoPlus Kit, 96 rxn9420053001YesYes
TRocheKAPA HyperCapture Reagent kit, 24 rxn9075810001YesYes
TRocheKAPA HyperCapture Reagent kit, 96 rxn9075828001YesYes
TRocheKAPA UDI Primer Mixes, 1-96 , 96 rxn9134336001YesYes
TRocheKAPA Unique Dual-Indexed Adapter PlateKK8726YesYes
NSeqWellplexWell 384PW384YesYes
TTakaraBioSMARTer Stranded RNA-Seq Kit, 12 Rxns634836YesYes
TTakaraBioSMARTer RNA Unique Dual Index Kit – 24U, 96 Rxns634451YesYes
TTwistTwist SARS-CoV-2 Research Panel, 12 Reactions, Kit102017YesYes
TTwistTwist Human Core Exome, 96 Reactions, Kit102027YesYes
TTwistTwist Universal Adapter System - TruSeq Compatible101308, 101309, 101310, 101311YesYes

* Requires amplification

Incompatible Kits

The following table lists kits that are not compatible with the Adept Workflow. Libraries generated with these kits require additional processing to increase circularization success. If your library prep or index kit is not listed, contact us for compatibility information.

CategoryVendorWorkflow
CustomQIAGENMultimodal Panels
NIlluminaBead Based Normalization
TNEBMethyl-seq Index
TNEBIndex-UDI Plate