What to know when considering a screen with Cytos
CRISPR and drug screens are great at finding hits, but figuring out why a hit happens usually means follow-up imaging, transcriptomics, and protein assays, each run on a different sample. Cytos brings morphology, protein, 3′ transcriptome, and guide RNA into a single screen on AVITI24™, linking every readout to the perturbation behind it.
This guide is for researchers evaluating Cytos for CRISPR screening, drug screening, or both. It covers how the workflow and chemistry work, what to consider when designing your screen, and what data and analysis tools to expect.
What you’ll learn
- Which CRISPR and drug screening applications Cytos supports, and which configuration fits your question
- How Direct In Sample Sequencing and fluorescence-based imaging chemistry capture RNA, morphology, and protein in the same cell
- Tips for evaluating your screen design
- What the data looks like and how to analyze it