AVITI™ OS 3.3.0 Release Notes

Please Note: We recommend users initiate the update during a period of instrument downtime. During the update process, the system may restart multiple times and will request a power cycle when the update is complete.

For systems with slower network connections and upgrading from AOS 2.x, the AOS 3.3.0 upgrade may take between 1-2 hours to complete. If the upgrade process does not complete within 3 hours, please contact ElemBio Support at support@elembio.com.

Summary of Changes

AVITI OS v3.3.0

For all instruments:

• Performance improvements to the system have reduced the priming times required before each sequencing run. For instruments below high-altitude, priming time is reduced to less than 5 minutes. For high-altitude instruments, priming time is reduced to less than 8 minutes. Users should be aware of this reduced priming time and stay near the instrument to initiate the run.
• Over-the-air update improvements. Update procedure requires user to power cycle the instrument prior to upgrade, if a power cycle has not been performed in the prior week.
• Air Filter Replacement Reminder: For high-elevation sites, a reminder is sent every 6 months. For other sites, it is sent once a year. Users can replace the filter and reset the timer through AVITIOS.
• When an ElemBio Catalyst™ subscription expires
  • Users will no longer be able to select the Catalyst storage connection during run setup.
  • The storage connection will be marked as expired in the system.

For AVITI and AVITI LT instruments:

• AVITI and AVITI LT instruments now require a maintenance wash once every 30 days to maintain performance of the instrument. A reminder will be displayed to instrument users 60 hours prior to the maintenance wash deadline, to allow planning ahead.

For AVITI24™ instruments:

• Support for New Fixed Teton™ Immuno-Oncology and Neuroscience Panels
  • New fixed Neuro and Immuno panels are now available in run setup.
  • Includes new cell segmentation models for suspension and neuronal cell types.
  • A small cell flag allows users to adjust amplification time for optimal signal detection.
• Support for New Teton Cartridge SKU
  • Users can now select the cartridge directly from the run setup screen.
  • The instrument UI has been updated to display the cartridge name throughout the workflow.
  • The prepare reagents screen now reminds users to load RNA and protein probe tubes into the cartridge.
  • The load reagents screen checks the cartridge part number against the selected cartridge.
• Support for Teton Custom Protein Add-On Panel
  • Users may load a Teton Custom Add-On Protein Panel from USB or a published cloud design.
  • The run setup screen now displays target names and validates panel content, ensuring:
    • The panel is a B08 barcoding batch with 5-88 barcoding targets.
    • The panel contains a valid panel name, ID, and probe concentrations.
  • The instrument UI now displays the custom panel name in the run summary.
  • When using an add-on custom panel, the prepare reagents screen prompts users to verify panel loading.
  • OBPA execution now uses a merged panel that includes both the selected fixed panel and the additional batch.
• Enhanced Telemetry Data Collection
  • RunStats.json and Panel.json files are collected.
  • A stitched projection of polonies across all tiles from the first cycle of each barcoding batch are collected.
• Telemetry enhancements to aid in remote troubleshooting
• New Metrics Collected
  • Nucleated rate – Percentage of cells with a nucleus.
  • Cellular assigned rate – (Polonies assigned to a barcode and inside a cell) / (Total polonies inside a cell).
  • Cellular polony dropout rate – Percentage of cells with no polonies in a batch for each well.
  • NuclearArea (nuclear area in pixels)
  • AreaUm (cellular area in microns)
  • NuclearAreaUm (nuclear area in microns)
  • XuM (X position in microns)
  • YuM (Y position in microns)
  • ZuM (Z position in microns relative to the Z00 slice)
  • Z (String label of the Z-slice, e.g. 00 or 01)

• These metrics are stored across run output files such as the RunStats.json, RawCellStats.csv, RawCellsStats.parquet, and {tile}_barcodes.parquet

Known Issues, v3.3.0:

• If an upgrade from AOS3.2.0 to AOS3.3.0 does not complete within two hours, users should reach out to support@elembio.com for additional support
• Percent Passing Filter metric may be displayed before final calculation is complete.
• If leak errors are triggered for both sides of the instrument, AVITI OS cannot unlock the waste module door.
• If the AFIC board loses power, it will not restart automatically and requires a power cycle. In the event of a power interruption or loss, power cycle the instrument via the UI after power is restored.
• The run can continue even if polony map generation fails, as AVITI OS cannot detect a particular failure scenario
• “NaN%” is displayed instead of “–” for the Q30 percentage of Library Pool 2 in dual library runs before it populates with the Q30 percentage.
• The RunParameters.json Recipe Values field does not include the cartridge part number and small cell value.
• Reagent and buffer part numbers are obscured on the Run Summary screen when a run includes a Custom Add-on Protein Panel.
• In some cases, z-slices of Cell Paint tiles may be missing, causing the projected image for that tile to appear fainter.