LAMP2

Protein Overview

LAMP2 is a heavily glycosylated lysosome-associated membrane protein that helps protect the lysosomal membrane and supports lysosome integrity, fusion, trafficking, and autophagic degradation. Specific LAMP2 isoforms also contribute to chaperone-mediated autophagy and specialized lysosomal functions in different tissues.

Expected Localization

Primarily lysosomal and late endosomal membranes.

In immunofluorescence, LAMP2 typically produces punctate cytoplasmic staining that may be concentrated in the perinuclear region, with minimal nuclear signal.

Expected Expression Pattern

Broadly expressed in nucleated mammalian cells, although abundance and lysosome number vary by cell type, differentiation state, metabolic activity, and autophagic or lysosomal stress. Strong expression is often observed in phagocytic and highly lysosome-rich cells.

Relevant Pathways

Lysosome organization and function
Macroautophagy and autophagosome-lysosome fusion
Chaperone-mediated autophagy
Endolysosomal trafficking
Cellular degradation and nutrient recycling

HeLa cells were cultured in a 12-well format Cytos slide kit overnight then fixed with 4% formaldehyde for 30 minutes, permeabilized using 70% ethanol for 10 minutes. The slide kit was then assembled into a flow cell and loaded onto an AVITI24™ along with the Cytos Protein Cartridge for a multiomics cell screening run. LAMP2 demonstrated strong lysosomal localization and high signal-to-noise ratio, with no detectable probe crosstalk. These results support high confidence in target-specific detection and validate this antibody as a passing clone. A549 cells were also cultured and processed under the same protocol in parallel as part of Cytos cross-run reproducibility testing.

Signal-to-noise ratio
Acceptance criteria: > 2x background

Pass

6.0 ± 1.9 (HeLa; n=26 runs)
6.2 ± 1.6 (A549; n=34 runs)

Indicates the fold brightness of the target signal as measured by the Mean Intensity Above (Otsu-defined) Threshold (IAT), over the background (a channel-matched isotype control) in the same cell. Data shown are mean ± standard deviation across independent reproducibility runs. The ratio ranges from 6.0–6.2 across HeLa and A549 cells, indicating that the signal is consistently about 3 times above the acceptance threshold — a clean, easily distinguishable signal.

Panel-level run-to-run reproducibility
Acceptance criteria: R² > 0.9

Pass

For each imaging panel, we assess reproducibility by comparing the intensity signal a given antibody target produces across independent replicate runs. Within each run, we measure the average per-well fluorescence intensity for every target in the panel. We then plot each target's intensity from one run against the matching target's intensity from another run of the same layout, and calculate the Pearson correlation coefficient (r) between those two sets of measurements. Squaring that value gives the R² (coefficient of determination), which reports what fraction of the variation in one run's signal is explained by the other run's signal.

Protein Overview

Clone Information

Qualitative Results

Quantitative Results

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