What types of cells can I run with Teton™ CytoProfiling Kits?

A variety of cell types can be run using Teton™. This knowledgebase article will be updated regularly as additional cell types are tested and validated. For foundational guidance on working with adherent and suspension cells, please refer to the Teton User Guide.

Additional articles are available to support users working specifically with adherent, suspension, or co-cultured cell types.

To further support sample preparation, the Teton Onboard Cell Paint Imaging Kit or Teton Cell Paint Probe Kit allow for the evaluation of sample quality, cell growth, and morphology—helping users refine their culture conditions and experimental variables. If you’re also interested in optimizing a custom protein panel and/or visualizing control protein probes, we recommend using the Teton Custom Antibody Screening Kit to evaluate sample quality, cell growth, morphology and polony counts within segmented cells.

We note the surface coatings for cell types ran in-house and observe that cells may change morphology with different coatings. For more information on optimizing your sample prep prior to a Teton run, please consult the Teton Optimization & Screening User Guide and the Teton Cell Culture Optimization Tech Note.

Validated Cell Types

These cell types have been run by Element Biosciences and have additional guidance including optimized segmentation algorithms.

Cell LineDB InfoTarget ConfluencyAdherent/SuspensionSurface CoatingsSegmentation ModelRecommendationsCell TypeSpecies
HCT116https://www.atcc.org/products/...50-70%AdherentPLLHCT116Use Small Cell Recipe if running AOS 3.3 or belowColonocyte, Large Intestine (Colorectal Carcinoma)Human
HeLahttps://www.atcc.org/products/ccl-250-70%AdherentPLL, Laminin, Matrigel, Collagen, Fibronectin or GelatinHeLaEpithelial, Cervix (Adenocarcenoma)Human
Hep G2https://www.atcc.org/products/...50-70%AdherentPLLHepG2These cells may be clumpy, ensure cells are in a single cell suspension prior to seedingEpithelial-Like, Liver (Hepatocellular Carcinoma)Human
HUVEC*https://www.atcc.org/products/pcs-100-01050-70%AdherentPLLHUVECPrimary Umbilical Vein Endothelial Cells (Normal)Human
Jurkat*https://www.atcc.org/products/...50-70%SuspensionPLLJurkatUse Small Cell Recipe if running AOS 3.3 or belowT Lymphoblast (Acute T Cell Leukemia)Human
MCF7https://www.atcc.org/products/...50-70%AdherentPLLMCF7Use Small Cell Recipe if running AOS 3.3 or below; These cells may be clumpy, ensure cells are in a single cell suspension prior to seedingEpithelial, Mammary Gland (Adenocarcinoma)Human
PC-3*https://www.atcc.org/products/...50-70%AdherentPLLPC-3Use Small Cell Recipe if running AOS 3.3 or belowEpithelial, Prostate (Grade IV Adenocarcinoma)Human
SH-Sy5yhttps://www.atcc.org/products/...30-50%AdherentLamininSH-Sy5yLaminin coating recommended when differentiatingEpithelial, Bone Marrow (Neuroblastoma)Human


Internally Tested Cell Types

These cell types have been run by Element Biosciences.

Cell LineDB InfoTarget ConfluencyAdherent/SuspensionSurface CoatingNotesCell TypeSpecies
697https://www.cellosaurus.org/CV...50-70%SuspensionPLLB acute lymphoblastic leukemiaHuman
22Rv1https://www.atcc.org/products/...50-70%AdherentPLLEpithelial, Prostate (Carcinoma)Human
A549https://www.atcc.org/products/...50-70%AdherentPLLEpithelial-Like, Lung (Carcinoma)Human
AstrocytesPrimary Culture50-70%Adherentcustom: matrigel
C4-2https://www.atcc.org/products/...50-70%AdherentPLLEpithelial-Like, Prostate (Prostate Cancer)Human
CACO2https://www.atcc.org/products/...50-70%AdherentPLLEnterocyte (Colorectal Adenocarcinoma)Human
DU-145https://www.atcc.org/products/...50-70%AdherentPLLEpithelial Prostate (Carcinoma)Human
HL-60https://www.atcc.org/products/ccl-24050-70%Suspensioncustom: PLLPromyeloblast (Acute promyelocytic leukemia)Human
HT-29https://www.atcc.org/products/...50-70%AdherentPLLEnterocyte (Colorectal Adenocarcenoma)Human
LNCaPhttps://www.atcc.org/products/...50-70%AdherentPLLEpithelial Prostate (Carcinoma)Human
MKN-45https://www.cellosaurus.org/CV...50-70%AdherentPLLGastric adenocarcenomaHuman
NALM6https://www.atcc.org/products/...50-70%SuspensionPLLLymphocyte-like, acute lymphoblastic leukemiaHuman
NKPrimary Culture50-70%SuspensionPLL
PANC1https://www.atcc.org/products/...50-70%AdherentPLLEpithelial, pancreatic duct (Carcinoma)Human
REHhttps://www.atcc.org/products/...50-70%SuspensionPLLLymphoblast, acute lymphocytic leukemiaHuman
RKOhttps://www.atcc.org/products/...50-70%AdherentPLLEpithelial Large Intestine, Colon (Carcinoma)Human
VCaPhttps://www.atcc.org/products/...50-70%AdherentPLLEpithelial, Prostate (Cancer)Human


Customer Run Cell Types

The list below contains cells that have been run in customer hands and are not listed in the validated or internally tested tables. Where possible we will share details about the success of these cell types and any tips/tricks for success.

Cell TypeDB InfoTarget ConfluencyRecommendationsCell TypeSpecies
GIST-T1https://www.cellosaurus.org/CV...50-70%Gastrointestinal Stromal Tumor Cell LineHuman
iPSCshttps://www.atcc.org/cell-prod...50-70%When using matrigel, titrate concentration to reduce lifting/peeling of cellsInduced Pluripotent Stem CellsHuman
HCC38https://www.atcc.org/products/...50-70%Epithelial Cell, Duct,Mammary Gland (TNM Stage IIB, grade 3 ductal carcinoma)Human
HCC1143https://www.atcc.org/products/...50-70%Epithelial Cell, Duct, Mammary Gland (TNM Stage IIA, grade 3 Ductal Carcinoma)Human
MDA-MB-231https://www.atcc.org/products/...50-70%Epithelial Cell, Mammary Gland (Adenocarcinoma)Human
BT-20https://www.atcc.org/products/...50-70%Epithelial Cell, Mammary Gland (Carcinoma)Human
HEK293https://www.atcc.org/products/...50-70%Epithelial, Kidney EmbryoHuman

This list will continually be updated, but for new cell types, please reach out to your Field Applications Scientist or to Element Support for our latest recommendations as you plan your experiment.

Things to avoid in your final sample prep

  • When working with gelatin-based custom coatings (eg gelatin, matrigel), ensure that you are not over-incubating. Over-incubating gelatin coatings can result in variable thickness and can lead to focus issues during the Teton run.
  • If purifying samples using magnetic beads, ensure that no magnetic beads transfer to the slide kit. The magnetic beads may dislodge during the course of the run and lead to fluidics concerns.

Please reach out to us if you are working with cells with endogenous fluorophores.


For more information, reach out to your Field Applications Scientist or Element Biosciences Support at support@elembio.com.

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