A variety of cell types can be run using Teton™. This knowledgebase article will be updated regularly as additional cell types are tested and validated. For foundational guidance on working with adherent and suspension cells, please refer to the Teton User Guide.
Additional articles are available to support users working specifically with adherent, suspension, or co-cultured cell types.
To further support sample preparation, the Teton Onboard Cell Paint Imaging Kit or Teton Cell Paint Probe Kit allow for the evaluation of sample quality, cell growth, and morphology—helping users refine their culture conditions and experimental variables. If you’re also interested in optimizing a custom protein panel and/or visualizing control protein probes, we recommend using the Teton Custom Antibody Screening Kit to evaluate sample quality, cell growth, morphology and polony counts within segmented cells.
We note the surface coatings for cell types ran in-house and observe that cells may change morphology with different coatings. For more information on optimizing your sample prep prior to a Teton run, please consult the Teton Optimization & Screening User Guide and the Teton Cell Culture Optimization Tech Note.
Validated Cell Types
These cell types have been run by Element Biosciences and have additional guidance including optimized segmentation algorithms.
| Cell Line | DB Info | Target Confluency | Adherent/Suspension | Surface Coatings | Segmentation Model | Recommendations | Cell Type | Species |
| HCT116 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | HCT116 | Use Small Cell Recipe if running AOS 3.3 or below | Colonocyte, Large Intestine (Colorectal Carcinoma) | Human |
| HeLa | https://www.atcc.org/products/ccl-2 | 50-70% | Adherent | PLL, Laminin, Matrigel, Collagen, Fibronectin or Gelatin | HeLa | Epithelial, Cervix (Adenocarcenoma) | Human | |
| Hep G2 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | HepG2 | These cells may be clumpy, ensure cells are in a single cell suspension prior to seeding | Epithelial-Like, Liver (Hepatocellular Carcinoma) | Human |
| HUVEC* | https://www.atcc.org/products/pcs-100-010 | 50-70% | Adherent | PLL | HUVEC | Primary Umbilical Vein Endothelial Cells (Normal) | Human | |
| Jurkat* | https://www.atcc.org/products/... | 50-70% | Suspension | PLL | Jurkat | Use Small Cell Recipe if running AOS 3.3 or below | T Lymphoblast (Acute T Cell Leukemia) | Human |
| MCF7 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | MCF7 | Use Small Cell Recipe if running AOS 3.3 or below; These cells may be clumpy, ensure cells are in a single cell suspension prior to seeding | Epithelial, Mammary Gland (Adenocarcinoma) | Human |
| PC-3* | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | PC-3 | Use Small Cell Recipe if running AOS 3.3 or below | Epithelial, Prostate (Grade IV Adenocarcinoma) | Human |
| SH-Sy5y | https://www.atcc.org/products/... | 30-50% | Adherent | Laminin | SH-Sy5y | Laminin coating recommended when differentiating | Epithelial, Bone Marrow (Neuroblastoma) | Human |
Internally Tested Cell Types
These cell types have been run by Element Biosciences.
| Cell Line | DB Info | Target Confluency | Adherent/Suspension | Surface Coating | Notes | Cell Type | Species |
| 697 | https://www.cellosaurus.org/CV... | 50-70% | Suspension | PLL | B acute lymphoblastic leukemia | Human | |
| 22Rv1 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial, Prostate (Carcinoma) | Human | |
| A549 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial-Like, Lung (Carcinoma) | Human | |
| Astrocytes | Primary Culture | 50-70% | Adherent | custom: matrigel | |||
| C4-2 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial-Like, Prostate (Prostate Cancer) | Human | |
| CACO2 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Enterocyte (Colorectal Adenocarcinoma) | Human | |
| DU-145 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial Prostate (Carcinoma) | Human | |
| HL-60 | https://www.atcc.org/products/ccl-240 | 50-70% | Suspension | custom: PLL | Promyeloblast (Acute promyelocytic leukemia) | Human | |
| HT-29 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Enterocyte (Colorectal Adenocarcenoma) | Human | |
| LNCaP | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial Prostate (Carcinoma) | Human | |
| MKN-45 | https://www.cellosaurus.org/CV... | 50-70% | Adherent | PLL | Gastric adenocarcenoma | Human | |
| NALM6 | https://www.atcc.org/products/... | 50-70% | Suspension | PLL | Lymphocyte-like, acute lymphoblastic leukemia | Human | |
| NK | Primary Culture | 50-70% | Suspension | PLL | |||
| PANC1 | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial, pancreatic duct (Carcinoma) | Human | |
| REH | https://www.atcc.org/products/... | 50-70% | Suspension | PLL | Lymphoblast, acute lymphocytic leukemia | Human | |
| RKO | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial Large Intestine, Colon (Carcinoma) | Human | |
| VCaP | https://www.atcc.org/products/... | 50-70% | Adherent | PLL | Epithelial, Prostate (Cancer) | Human |
Customer Run Cell Types
The list below contains cells that have been run in customer hands and are not listed in the validated or internally tested tables. Where possible we will share details about the success of these cell types and any tips/tricks for success.
| Cell Type | DB Info | Target Confluency | Recommendations | Cell Type | Species |
| GIST-T1 | https://www.cellosaurus.org/CV... | 50-70% | Gastrointestinal Stromal Tumor Cell Line | Human | |
| iPSCs | https://www.atcc.org/cell-prod... | 50-70% | When using matrigel, titrate concentration to reduce lifting/peeling of cells | Induced Pluripotent Stem Cells | Human |
| HCC38 | https://www.atcc.org/products/... | 50-70% | Epithelial Cell, Duct,Mammary Gland (TNM Stage IIB, grade 3 ductal carcinoma) | Human | |
| HCC1143 | https://www.atcc.org/products/... | 50-70% | Epithelial Cell, Duct, Mammary Gland (TNM Stage IIA, grade 3 Ductal Carcinoma) | Human | |
| MDA-MB-231 | https://www.atcc.org/products/... | 50-70% | Epithelial Cell, Mammary Gland (Adenocarcinoma) | Human | |
| BT-20 | https://www.atcc.org/products/... | 50-70% | Epithelial Cell, Mammary Gland (Carcinoma) | Human | |
| HEK293 | https://www.atcc.org/products/... | 50-70% | Epithelial, Kidney Embryo | Human |
This list will continually be updated, but for new cell types, please reach out to your Field Applications Scientist or to Element Support for our latest recommendations as you plan your experiment.
Things to avoid in your final sample prep
- When working with gelatin-based custom coatings (eg gelatin, matrigel), ensure that you are not over-incubating. Over-incubating gelatin coatings can result in variable thickness and can lead to focus issues during the Teton run.
- If purifying samples using magnetic beads, ensure that no magnetic beads transfer to the slide kit. The magnetic beads may dislodge during the course of the run and lead to fluidics concerns.
Please reach out to us if you are working with cells with endogenous fluorophores.