The power of the LoopSeq approach stems from our unique, proprietary barcoding technology and implementation. The key steps of the workflow are described below.
Every sample is exposed to millions of unique barcodes, but only one barcode attaches per strand of DNA.
Every molecule, along with its unique barcode, is amplified using PCR.
Each copy of the amplified DNA has the barcode randomly distributed to a different location.
Sequence the segment next to each barcode.
Short reads that share the same barcode are combined algorithmically into a full-length molecule using linked- read de novo assembly.
LoopSeq PCR Amplicon and 16S SSC Workflows
The LoopSeq PCR Amplicon Workflow prepares libraries for long-read sequencing on traditional short-read sequencing platforms or the Element AVITI™ System, making Element the only company to offer both short- and long-read sequencing.
Offering general-purpose amplicon library prep across disciplines, the PCR amplicon workflow is a two-kit protocol adds unique molecular identifiers (UMIs) pre-PCR to eliminate bias and multiplexes up to 24 samples in one tube for streamlined library prep. Visit the Loop Genomics website for more information on our long-read solution and compatible applications.
Complementing the broad spectrum of applications that PCR amplicon supports, the LoopSeq 16S SSC Workflow targets a bacterial population of microbiome samples for comprehensive profiling based on all nine variable regions. The workflow features a similar UMI-based, multiplexing protocol as the PCR amplicon workflow. Both workflows use the LoopSeq Core Construction Kit for library prep.
|Specification||LoopSeq PCR Amplicon Kit||LoopSeq 16S SSC Kit|
|Storage temperature||-25°C to -15°C||25°C to -15°C|
|Input requirement||10 ng genomic DNA (gDNA) (2 ng/µl)||10 ng gDNA (2 ng/µl)|
|Specialized sample types||Genomes, metagenomes, PCR products, synthetic DNA libraries, viruses||Microbial|
|Size range||PCR fragments ≤ 4 Kb||Not applicable|
~8.5 hours~2.5 hours
|Multiplexing||Process ≤ 24 samples in one tube||Process ≤ 96 samples in one tube|
|Sequencing||Element AVITI System and short-read sequencing platforms|
2 x 151 paired-end (PE)
|Element AVITI System and short-read sequencing platforms|
2 x 151 PE
The Element Adept™ Library Compatibility Workflow adapts linear libraries prepared with third-party kits for sequencing on the Element AVITI System. Leveraging a custom splint mix that adds Element surface primers to compatible libraries, the workflow grants the benefit of adapting a wide variety of existing libraries—including all LoopSeq synthetic long-read kits available today.
As a single-kit workflow, the Element Adept Compatibility Kit is simple to use and produces high-quality libraries with highly consistent results between sequencing runs. Visit Adept Library Compatibility Kit for more details.