Summary of Changes
AVITI OS v2.4.1 (Patch release to v2.4.0)
- To address potential run failure if demultiplexing barcodes for index1 and index2 are different lengths.
AVITI OS v2.4.0
Comprehensive support for new sequencing kits:
- AVITI™ 2x300 Sequencing Kit Cloudbreak™ High Output
- AVITI™ 2x300 Sequencing Kit Cloudbreak™ Medium Output
Support for Low-Diversity Amplicon Libraries:
- When you set up a run for the Adept Workflow and a 2 x 300 sequencing kit, you can enable a Low-Diversity Amplicon Library option. Enabling this option requires high sample index plexity but reduces the amount of PhiX spike-in required to sequence 16S amplicons and other low-diversity libraries. The option therefore increases throughput and decreases cost.
Support for the AVITI System LT:
- Visual indicators differentiate between the full AVITI System and the AVITI System LT, including new logos, new home screen background colors, and new start-up screens.
- AVITI OS limits sequencing kit support for the AVITI System LT to the following medium and low output kits:
- AVITI™ 2x300 Sequencing Kit Cloudbreak™ Medium Output
- AVITI™ 2x150 Sequencing Kit Cloudbreak™ Medium Output
- AVITI™ 2x150 Sequencing Kit Cloudbreak™ Low Output
- AVITI™ 2x75 Sequencing Kit Cloudbreak™ Medium Output
- AVITI™ 2x300 Sequencing Kit Cloudbreak™ Medium Output
- High Output Kits Add-On
- The High Output Kits add-on appears on the full AVITI System and any AVITI System LT that upgrades to the full AVITI system. The add-on lets users select high-output sequencing kits in addition to medium and low- output kits when setting up a sequencing run.
Sequencing kit output levels appear for planned runs:
- When applicable, sequencing kit output levels appear for planned runs on the instrument.
Sustaining improvements:
- If one library pool fails to generate data for a run that uses individually addressable lanes, the software now notifies the user that data is unavailable for the failed library pool.
- For High Elevation mode, run setup estimates priming times more precisely than before.
- Instrument start-up includes additional error handling for rare nest hardware failures.
- Updates improve the process for software and firmware upgrades.
- The Notifications list now includes pagination.
Known Issues:
- If the waste module leak errors are triggered for both instrument sides, the user cannot unlock the waste module door.
- Low diversity runs can cause the thumbnail to have a green or yellow hue; an upcoming release will address this issue.