Modern agricultural breeding programs increasingly rely on genotyping data as a fundamental component of genomic selection models. Common methods for acquiring this data include whole exome/hybrid capture enrichment, genotyping arrays, and, more recently, low-pass whole genome sequencing, each with distinct advantages and limitations. Here, we introduce a novel approach leveraging a streamlined combination of target capture and low pass WGS designed to overcome those limitations while taking advantage of the scale and reduced cost of next generation sequencing.
Download the poster to see how with Trinity™ from Element Biosciences you can combine a targeted set of trait-linked markers with low-pass WGS to enable further discovery across the genome. Highlights include:
- A reduction in targeted library preparation time from over 20 hours to as little as 5 hours with significant reduction in hands-on time by automating capture and washing steps directly on flow cell
- An introduction to a modified Trinity protocol enabling the addition of background whole genome data at a user-defined coverage level
- Results from a use case showing high coverage uniformity from 43K targeted regions across the peanut genome combined with a 1.5x genome using a single library