Count On Element Biosciences to Make Single-Cell RNA Sequencing More Affordable

Single-cell RNA sequencing has rapidly become a critical tool for scientists who study tissues where transcriptional diversity drives biological functions, including immunology, neuroscience, cancer, stem cell research, and developmental biology. Single-cell sequencing is a relatively new tool with many open challenges, including dropouts and the need for new data science methods, but managing the cost of experiments is almost always a significant consideration.

Here, the AVITI™ System represents a new, disruptive force in next-generation sequencing (NGS) technology that offers exceptional DNA sequencing accuracy at a fraction of the typical reagent cost. By leveraging low-binding flow cell surface chemistry, polony generation with rolling-circle amplification, and our proprietary Avidite technology, the AVITI System generates base calling accuracy that exceeds 90% Q30. And it does so at a cost per gigabase on par with factory-level sequencing, without factory-scale batch requirements.

Element introduces a new 2x75 paired-end sequencing kit for RNA sequencing

To bring all these benefits to scientists who use RNA and single-cell RNA sequencing, Element has now released a 2x75 paired-end sequencing kit. With 1 billion reads per flow cell at an operating cost of just ~$1 per million reads, AVITI will let you run more experiments, sequence more cells, or sequence more deeply so you can do more science with your budget.

Brief Overview of Our New 2x75 Sequencing Kits by Isaku Tanida, PhD, Associate Director of Product Marketing

The AVITI 2x75 sequencing kit is a nominal 150-cycle reagent kit that can be used for SE or PE sequencing with an actual cycle capacity of 184 cycles to allow for sequencing both index and bar code sequences. Identical to the larger AVITI 2x150 Sequencing kit, the AVITI 2x75 Sequencing kit offers the same 1B read capacity, making it ideal for counting assays on a cost-per-read basis.

The AVITI System joins the 10X Genomics Compatibility Partner Program

AVITI fits seamlessly within the NGS ecosystem, as shown through its many application partnerships, including 10X Genomics. In fact, Element is only the second-ever member of the 10X Genomics Compatibility Partner Program for Chromium Single Cell 3’ Assays.

Figure 1: Summary of the 10x Chromium Single Cell workflow using the Element AVITI System

As part of the partner program validation, the AVITI system was used to sequence a control PBMC sample at both small (1,000 cells) and large (10,000) population scales. Data from the AVITI system met or exceeded all required CPP performance criteria for both scales, including standards for barcode validity, mapped reads, reproducibility, and mean reads per cell. You can learn more details by downloading and reading our application note.

New tools for custom library preparation with the Adept Library Compatibility Kit v1.1.

At Element, increasing access to sequencing goes beyond affordability – we believe that flexibility is just as important. To provide even greater flexibility within the AVITI workflow, Element is now enabling users to pursue custom library sequencing through its launch of a new Adept Library Compatibility Kit v1.1 and a new Adept Custom Oligonucleotide Buffer Set kit.

Adept v1.1 features a universal qPCR solution that enables the quantitation of custom libraries before sequencing, as is currently enabled for standard AVITI System workflows. The corresponding Adept Custom Oligonucleotide Buffer Set kit allows users to introduce custom primers in a separate buffered solution set vs. a spike-in approach. This means custom primers no longer need to be mixed with primers from the standard workflow.

Be on the lookout for more exciting new products that further extend the capabilities of the AVITI System; the best investment in a sequencing workflow you can make.

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*Read count based on Element control library sequencing. Actual read count might differ based on library type, library preparation, and other factors.