Element Elevate™ Library Prep Workflow
The Element Elevate Library Prep Workflow offers native library prep solutions for a wide range of applications, from whole-genome sequencing (WGS) and other DNA sequencing applications to RNA sequencing (RNA-Seq). Built on a foundation of efficient recovery and consistent results, the Elevate Workflow accommodates variable sample types and input amounts, including low input and low quality. Efficient, user-friendly protocols with onboard circularization minimize touchpoints and accelerate library prep time.
Go End-to-End with Elevate
The Elevate Workflow features a modular kit design for greater flexibility. Mix and match library prep, enrichment, and index kits to configure your ideal end-to-end prep. Workflow choices include:
- DNA library prep with or without PCR
- Mechanical or enzymatic fragmentation of DNA samples
- Messenger RNA (mRNA) capture or ribosomal RNA (rRNA) depletion for enrichment of RNA samples
- Multiplexing of up to 96 samples with 96 unique dual indexes (UDIs) for accurate read assignment
- Input optimizations for RNA integrity numbers (RINs) both above and below 7
- Standalone index and adapter plates for custom library prep
- Size selection that calibrates insert sizes
Elevate Specifications
Built to stringent specifications to support a variety of study requirements, DNA and RNA library prep kits prepare libraries for avidity base chemistry sequencing in less than four hours for DNA libraries and about six hours for RNA libraries. For complete compatibility information, see Product Compatibility.
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Specification | Mechanical Kit | Enzymatic Kit | RNA Kit |
Kit size | 24 reactions | 24 reactions | 24 reactions |
Multiplexing | ≤ 96 UDIs | ≤ 96 UDIs | ≤ 96 UDIs |
Automation capability | Liquid handling robot | Liquid handling robot | Liquid handling robot |
Input requirement | 1–500 ng sheared genomic (gDNA) | 1–500 ng gDNA | 10–4000 ng RNA |
Fragmentation method | Covaris shearing | Enzymatic | Enzymatic |
Enrichment method | Not applicable | Not applicable | mRNA enrichment or rRNA depletion |
PCR-free protocol time* | 2 hours and 25 minutes | 1 hour and 50 minutes | Not applicable |
PCR-plus protocol time* | 3 hours and 25 minutes | 2 hours and 55 minutes | 6 hours |
Storage | -25°C to -15°C | -25°C to -15°C | -25°C to -15°C |
* Approximate total time that includes hands-on time
Catalog Numbers
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Product | Catalog # |
DNA Library Prep | |
Elevate Mechanical Library Prep Kit | 830-00008 |
Elevate Enzymatic Library Prep Kit | 830-00009 |
Elevate Long UDI Adapter Kit Set A | 830-00010 |
Elevate Index and Adapter Kit | 830-00005 |
RNA Library Prep | |
Elevate RNA Library Prep Kit | 830-00019 |
Elevate mRNA Capture Kit for RNA Library Prep | 830-00021 |
Elevate rRNA Depletion Kit for RNA Library Prep | 830-00022 |
Elevate RNA Index and Adapter Kit | 830-00020 |