
The Trinity Freestyle™ workflow combines the speed and simplicity you expect from Trinity™ with expanded flexibility. It brings together a streamlined 1 hour hybridization and an effortless fit into your current pipeline, offering universal P5/P7 library compatibility through Twist EF2.0 and Twist FlexPrep.
Built for speed and flexibility
- Sample to sequencer in as little as five hours—Accelerate your workflow with a 1 hour hybridization and eliminate time-consuming post-capture bead washing, PCR, and QC steps.
- Seamless integration—Easily fit into your existing pipelines with seamless compatibility for any Twist sequencing library using P5 and P7 adapters.
- Efficiency at scale – Minimize hands-on time and increase throughput for greater productivity.
- Flexibility across applications – From oncology and clinical research to agbio and population genomics.

Seamless integration for every application
Trinity Freestyle connects with Twist Enzymatic Fragmentation 2.0 and FlexPrep UHT kits, expanding flexibilty across research areas from whole exome sequencing to oncology and agbio genotyping.
- Oncology and tumor profiling—Pair with Twist EF2.0 for precise, rapid hybrid capture
- High-throughput genotyping—Accelerate workflows for highly multiplexed genotyping for agbio or population genomics with Twist FlexPrep UHT
- Expanding support—Ongoing validation continues to broaden compatibility and applications.
Don’t wait to simplify your workflow
Whether you joined the webinar or are learning about Trinity Freestyle for the first time, see how this collaboration can transform your targeted sequencing.