Need help in the lab? We've got you covered.
Element provides a service lab for LoopSeq. It’s as simple as sending
your extracted DNA or RNA samples to us and we’ll get the data and a
full computational analysis back to you and your team.
Long-Read Services Provided | |
| 16S rRNA Gene Sequencing | 16S/18S rDNA Sequencing |
| Mycobiome Sequencing | Transcriptome Sequencing/Profiling |
| DNA (Amplicon, PCR) Sequencing | Single Cell Transcriptome Sequencing/Profiling |
| Bacterial Whole Genome Sequencing | Solo Clone & Isolate Sequencing |
| Immune Repertoire Sequencing |
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Industry Experience
We have worked across the following sectors...
16S/18S rRNA Gene Sequencing and Data Analysis
Service description: We will use our LoopSeq single molecule-counting long-read 16S/18S Ribosomal DNA kit to sequence your microbiome samples to determine the type and relative abundance of bacterial and archaeal species. We can accept purified DNA from gut, skin, soil, marine or other sources.
Advantages:
- Higher sensitivity than other 16s/18s services as we interrogate the entire 16s/18s molecule V1-V9 with our single molecule counting long-read technology
- 40x lower error when compared to Illumina library prep
- Cost-effective
- Fast turnaround
Region Coverage: Full 16s/18s molecules, all V1-V9 variable regions
Deliverables: Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S and 18S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.
Sample Submission: High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin
Mycobiome - 18S ITS1 & ITS2 rRNA Gene Sequencing and Data Analysis
Service Description: We will use our LoopSeq single molecule-counting long-read Mycobiome 18S ITS1 & ITS2 Ribosomal RNA kit to sequence your mycobiome samples to determine the type and relative abundance of fungal species. We can accept purified DNA from gut, skin, soil, marine or other sources.
Advantages:
- Higher sensitivity than other services as we interrogate the 18S ITS1 & ITS2 region and the same time with our single molecule counting long-read technology
- 40x lower error when compared to Illumina library prep
- Cost-effective
- Fast turnaround
Region Coverage: 2.5Kb contiguous 18S ITS1 & ITS2
Deliverables: Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S and 18S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.
Sample Submission: High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin
Transcriptome Sequencing and Profiling
Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology for Transcriptome sequencing of your samples. Unlike RNA-seq which only counts transcripts we will not only provide transcript counting results but also unique isoform detection, enabled by our long-read technology.
Advantages:
- Choose isoform discovery, transcript abundance measurement, or both
- 40x lower error when compared to Illumina library prep, 7,500x lower error when compared to PacBio
- Cost-effective
- Fast turnaround
Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed mRNA long reads. Additionally, each RNA sample includes a downstream analysis summarized in a CSV file that includes (1) Single molecule quantification of mRNA abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases and providing accurate gene expression quantification, (2) Alignment of the mRNA long reads to RefSeq and a complete statistical report on each aligned molecule. Additionally, alignment to custom databases is available upon request.
Sample Submission: 10 ng of total RNA
Single Cell Transcriptome Sequencing and Profiling
Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology for Transcriptome sequencing of your single-cell samples. Unlike RNA-seq which only counts transcripts we will not only provide transcript counting results but also unique isoform detection, enabled by our long-read technology.
Advantages:
- Choose isoform discovery, transcript abundance measurement, or both
- 40x lower error when compared to Illumina library prep, 7,500x lower error when compared to PacBio
- Cost-effective
- Fast turnaround
Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed mRNA long reads. Additionally, each RNA sample includes a downstream analysis summarized in a CSV file that includes (1) Single molecule quantification of mRNA abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases and providing accurate gene expression quantification, (2) Alignment of the mRNA long reads to RefSeq and a complete statistical report on each aligned molecule. Additionally, alignment to custom databases is available upon request.
Sample Submission: 10 ng of total RNA
Optional Long-Read Probe Capture: Focus your sequencing results with optional long-read probe capture.
DNA (Amplicon, PCR) Sequencing and Profiling
Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology to for DNA sequencing of your samples. We will not only provide single-molecule counting results for true abundance but provide long-read output as enable by our unique technology. Input is any dsDNA. Input can be a long-range PCR product, Amplicon, etc.
Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed DNA long reads from the barcoded short treads. Additionally, each sample includes downstream analysis summarized in a CSV files with alignment of each reconstructed DNA molecule and a reference database.
Sample Submission: 10 ng of and dsDNA, long-range PCR product, amplicon, etc.
Bacterial Whole Genome Sequencing and Assembly
Service Description: Microbial genome long-read sequencing service using LoopSeq library preparation. De novo long-read sequencing is performed without prior knowledge of the samples. We will deliver long-read FASTA and FASTQ files and do sequence assembly. Your samples will be sequenced on an NGS platform using paired-end 150 bp.
Advantages:
- Cost-effective
- 40x lower error rate than traditional Illumina sequencing
- Greater accuracy with bias-free single-molecule counting
- Fast turnaround
Deliverables: FASTA, FASTQ and bioinformatics analysis
Mean Depth of Coverage: 100x for genomes ~5Mb in size (Higher coverage available for additional charges, please inquire)
Sample Submission: High quality genomic DNA: a minimum of 10 nanogram as quantified by Qubit 2.0
Turnaround Time: 3 weeks
Solo Clone & Isolate Long-Read Sequencing and Analysis
Ultra-accurate, full-length Element LoopSeq sequencing service is more cost-effective than Sanger sequencing & next-generation sequencing at high numbers of samples.
Immune Repertoire Long-Read Sequencing
Understand the diversity of the immune cell repertoire with full-length, single molecule sequencing of B cell receptors, T cell receptors, immunoglobulins, and scFvs
All services provided are intended for research purposes only - terms & conditions apply.