Element LoopSeq™ Long-Read Sequencing Services

Need help in the lab? We've got you covered.

Element also provides a service lab for LoopSeq. It’s as simple as sending your extracted DNA or RNA samples to us and we’ll get the data and a full computational analysis back to you and your team.

Industry Experience

We have worked across the following sectors...

Interested in a Service Project?

16S rRNA Gene Sequencing16S/18S rDNA Sequencing
Mycobiome SequencingTranscriptome Sequencing/Profiling
DNA (Amplicon, PCR) SequencingSingle Cell Transcriptome Sequencing/Profiling
Bacterial Whole Genome SequencingSolo Clone & Isolate Sequencing
Immune Repertoire Sequencing

16S rRNA Gene Sequencing and Data Analysis

Service description: We will use our LoopSeq single molecule-counting long-read 16S Ribosomal DNA kit to sequence your microbiome samples to determine the type and relative abundance of bacterial and archaeal species. We can accept purified DNA from gut, skin, soil, marine or other sources.

Advantages:

  • Higher sensitivity than other 16s services as we interrogate the entire 16s molecule V1-V9 with our single molecule counting long-read technology
  • 40x lower error when compared to Illumina library prep
  • Cost-effective
  • Fast turnaround

Region Coverage: Full 16s molecule, all V1-V9 variable regions
Deliverables: Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.
Turnaround Time: 4 weeks
Sample Submission: High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin
Minimum Number of Samples: 8 (Learn about high-volume pricing here)

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16S/18S rRNA Gene Sequencing and Data Analysis

Service description: We will use our LoopSeq single molecule-counting long-read 16S/18S Ribosomal DNA kit to sequence your microbiome samples to determine the type and relative abundance of bacterial and archaeal species. We can accept purified DNA from gut, skin, soil, marine or other sources.

Advantages:

  • Higher sensitivity than other 16s/18s services as we interrogate the entire 16s/18s molecule V1-V9 with our single molecule counting long-read technology
  • 40x lower error when compared to Illumina library prep
  • Cost-effective
  • Fast turnaround

Region Coverage: Full 16s/18s molecules, all V1-V9 variable regions
Deliverables: Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S and 18S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.
Turnaround Time: 3 weeks
Sample Submission: High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin
Minimum Number of Samples: 8

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Mycobiome - 18S ITS1 & ITS2 rRNA Gene Sequencing and Data Analysis

Service Description: We will use our LoopSeq single molecule-counting long-read Mycobiome 18S ITS1 & ITS2 Ribosomal RNA kit to sequence your mycobiome samples to determine the type and relative abundance of fungal species. We can accept purified DNA from gut, skin, soil, marine or other sources.

Advantages:

  • Higher sensitivity than other services as we interrogate the 18S ITS1 & ITS2 region and the same time with our single molecule counting long-read technology
  • 40x lower error when compared to Illumina library prep
  • Cost-effective
  • Fast turnaround

Region Coverage: 2.5Kb contiguous 18S ITS1 & ITS2
Deliverables: Our standard analysis includes CSV files with (1) Single molecule quantification of abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases, (2) Reference based taxonomic classification based on nine variable regions (V1-V9 data in 16S and 18S) and long-reads in Metagenomic sequencing, (3) OTU tables, (4) Rarefaction analysis, (5) RAD analysis, as well as pie chart visualizations of classification and quantification.(5) Raw data as FASTA and FASTQ files.
Turnaround Time: 3 weeks
Sample Submission: High quality genomic DNA , a minimum of 10ng as quantified by Qubit 2.0, or 1ng DNA from skin
Minimum Number of Samples: 8

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Transcriptome Sequencing and Profiling

Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology for Transcriptome sequencing of your samples. Unlike RNA-seq which only counts transcripts we will not only provide transcript counting results but also unique isoform detection, enabled by our long-read technology.

Advantages:

  • Choose isoform discovery, transcript abundance measurement, or both
  • 40x lower error when compared to Illumina library prep, 7,500x lower error when compared to PacBio
  • Cost-effective
  • Fast turnaround

Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed mRNA long reads. Additionally, each RNA sample includes a downstream analysis summarized in a CSV file that includes (1) Single molecule quantification of mRNA abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases and providing accurate gene expression quantification, (2) Alignment of the mRNA long reads to RefSeq and a complete statistical report on each aligned molecule. Additionally, alignment to custom databases is available upon request.
Sample Submission: 10 ng of total RNA
Minimum Number of Samples: 8

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Single Cell Transcriptome Sequencing and Profiling

Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology for Transcriptome sequencing of your single-cell samples. Unlike RNA-seq which only counts transcripts we will not only provide transcript counting results but also unique isoform detection, enabled by our long-read technology.

Advantages:

  • Choose isoform discovery, transcript abundance measurement, or both
  • 40x lower error when compared to Illumina library prep, 7,500x lower error when compared to PacBio
  • Cost-effective
  • Fast turnaround

Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed mRNA long reads. Additionally, each RNA sample includes a downstream analysis summarized in a CSV file that includes (1) Single molecule quantification of mRNA abundance based on Unique molecular Identifiers (UMI's), eliminating amplification biases and providing accurate gene expression quantification, (2) Alignment of the mRNA long reads to RefSeq and a complete statistical report on each aligned molecule. Additionally, alignment to custom databases is available upon request.
Sample Submission: 10 ng of total RNA
Minimum Number of Samples: 8
Optional Long-Read Probe Capture: Focus your sequencing results with optional long-read probe capture.

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DNA (Amplicon, PCR) Sequencing and Profiling

Service Description: We will use our LoopSeq single-molecule counting long-read sequencing technology to for DNA sequencing of your samples. We will not only provide single-molecule counting results for true abundance but provide long-read output as enable by our unique technology. Input is any dsDNA. Input can be a long-range PCR product, Amplicon, etc.
Deliverables: Our standard analysis includes FASTQ and FASTA files with the reconstructed DNA long reads from the barcoded short treads. Additionally, each sample includes downstream analysis summarized in a CSV files with alignment of each reconstructed DNA molecule and a reference database.
Sample Submission: 10 ng of and dsDNA, long-range PCR product, amplicon, etc.
Minimum Number of Samples: 8

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Bacterial Whole Genome Sequencing and Assembly

Service Description: Microbial genome long-read sequencing service using LoopSeq library preparation. De novo long-read sequencing is performed without prior knowledge of the samples. We will deliver long-read FASTA and FASTQ files and do sequence assembly. Your samples will be sequenced on an NGS platform using paired-end 150 bp.

​RESEARCH USE ONLY

Advantages:

  • Cost-effective
  • 40x lower error rate than traditional Illumina sequencing
  • Greater accuracy with bias-free single-molecule counting
  • Fast turnaround

Deliverables: FASTA, FASTQ and bioinformatics analysis
Mean Depth of Coverage: 100x for genomes ~5Mb in size (Higher coverage available for additional charges, please inquire)
Sample Submission: High quality genomic DNA: a minimum of 10 nanogram as quantified by Qubit 2.0
Turnaround Time: 3 weeks
Minimum Number of Samples: 3

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Solo Clone & Isolate Long-Read Sequencing and Analysis

Ultra-accurate, full-length Element LoopSeq sequencing service is more cost-effective than Sanger sequencing & next-generation sequencing at high numbers of samples.

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Immune Repertoire Long-Read Sequencing

Understand the diversity of the immune cell repertoire with full-length, single molecule sequencing of B cell receptors, T cell receptors, immunoglobulins, and scFvs

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